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The first cloned sea cucumber FADD from Holothuria leucospilota: Molecular characterization, inducible expression and involvement of apoptosis
Zhao, Lin; Ren, Chunhua1; Chen, Ting1; Sun, Hongyan2; Wu, Xiaofen1; Jiang, Xiao1; Huang, Wen1
2019
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume89Pages:548
AbstractIn this study, a sea cucumber Fas-associated death domain (FADD) named HLFADD was first cloned from Holothuria leucospilota. The full-length cDNA of HLFADD is 2137 bp in size, containing a 116-bp 5'-untranslated region (UTR), a 1334-bp 3'-UTR and a 687-bp open reading frame (ORF) encoding a protein of 228 amino acids with a deduced molecular weight of 26.42 kDa. HLFADD protein contains a conserved death effector domain at its N-terminal and a conserved death domain at its C-terminal, structurally similar to its counterparts in vertebrates. The over-expressed HLFADD protein could induce apoptosis in HEK293 cells, suggesting a possible death receptor-mediated apoptosis pathway in echinoderms adapted with FADD. Moreover, HLFADD mRNA is ubiquitously expressed in all examined tissues, with the highest transcript level in the coelomocytes, followed by intestine. In vitro experiments performed in the H. leucospilota coelomocytes, the expression of HLFADD mRNA was significantly up-regulated by lipopolysaccharides (LPS) or polyriboinosinic-polyribocytidylic acid [poly (I:C)] challenge, suggesting that HLFADD might play important roles in the innate immune defense of sea cucumber against the invasion of bacteria and viruses.
DepartmentLMB
KeywordFas-associated death domain Holothuria leucospilota Apoptosis Innate immune
DOI10.1016/j.fsi.2019.04.030
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17835
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Guangdong Pharmaceut Univ, Sch Biosci & Biopharmaceut, Guangdong Prov Key Lab Biotechnol Candidate Drug, Guangzhou 510006, Guangdong, Peoples R China
2.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, Guangzhou 510301, Guangdong, Peoples R China
3.South China Agr Univ, Coll Marine Sci, Joint Lab Guangdong Prov & Hong Kong Reg Marine B, Guangzhou 510642, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Lin,Ren, Chunhua,Chen, Ting,et al. The first cloned sea cucumber FADD from Holothuria leucospilota: Molecular characterization, inducible expression and involvement of apoptosis[J]. FISH & SHELLFISH IMMUNOLOGY,2019,89:548, 554.
APA Zhao, Lin.,Ren, Chunhua.,Chen, Ting.,Sun, Hongyan.,Wu, Xiaofen.,...&Huang, Wen.(2019).The first cloned sea cucumber FADD from Holothuria leucospilota: Molecular characterization, inducible expression and involvement of apoptosis.FISH & SHELLFISH IMMUNOLOGY,89,548.
MLA Zhao, Lin,et al."The first cloned sea cucumber FADD from Holothuria leucospilota: Molecular characterization, inducible expression and involvement of apoptosis".FISH & SHELLFISH IMMUNOLOGY 89(2019):548.
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