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Characterization of one novel microbial esterase WDEst9 and its use to make l-methyl lactate
Wang, Yilong1; Xu, Shan2; Li, Renqiang2; Sun, Aijun1; Zhang, Yun1; Sai, Ke3,4,5; Hu, Yunfeng1
2019
Source PublicationBIOCATALYSIS AND BIOTRANSFORMATION
ISSN1024-2422
Volume37Issue:3Pages:190
AbstractChiral lactic acids and their ester derivatives are crucial building blocks and intermediates for the synthesis of a great variety of valuable functional materials and pharmaceuticals. Before our study, the reports about the enantioselective preparation of pure L-lactic acid and its ester derivatives through direct hydrolysis of racemic substrate were quite rare. Herein, we heterologously expressed and functionally characterized one novel microbial esterase WDEst9 from Dactylosporangium aurantiacum, which exhibited high resistance to diverse metal ions, organic solvents, surfactants, NaCl and KCl. We further utilized WDEst9 as a green biocatalyst in the kinetic resolution of (+/-)-methyl lactate through direct hydrolysis and generated L-methyl lactate with high enantiomeric excess (e.e. >99%) and high yield (>86%) after process optimization. Notably, the enantioselectivity of WDEst9 was opposite than that of two previously reported esterases PHE14 and BSE01701 that can generate D-methyl lactate though kinetic resolution of (+/-)-methyl lactate. Microbial esterase WDEst9 is a promising green biocatalyst in the preparation of valuable chiral chemicals and opens the door for the identification of useful industrial enzymes and biocatalysts from the genus Dactylosporangium.
DepartmentLMB
KeywordBiocatalysis novel esterase kinetic resolution L-methyl lactate opposite enantioselectivity
DOI10.1080/10242422.2018.1526926
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17848
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China
2.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Materia Med, Guangzhou, Guangdong, Peoples R China
3.Jinan Univ, Dept Biotechnol, Guangzhou, Guangdong, Peoples R China
4.Sun Yat Sen Univ, Canc Ctr, Dept Neurosurg Neurooncol, Guangzhou, Guangdong, Peoples R China
5.Collaborat Innovat Ctr Canc Med, Guangzhou, Guangdong, Peoples R China
6.State Key Lab Oncol South China, Guangzhou, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yilong,Xu, Shan,Li, Renqiang,et al. Characterization of one novel microbial esterase WDEst9 and its use to make l-methyl lactate[J]. BIOCATALYSIS AND BIOTRANSFORMATION,2019,37(3):190, 200.
APA Wang, Yilong.,Xu, Shan.,Li, Renqiang.,Sun, Aijun.,Zhang, Yun.,...&Hu, Yunfeng.(2019).Characterization of one novel microbial esterase WDEst9 and its use to make l-methyl lactate.BIOCATALYSIS AND BIOTRANSFORMATION,37(3),190.
MLA Wang, Yilong,et al."Characterization of one novel microbial esterase WDEst9 and its use to make l-methyl lactate".BIOCATALYSIS AND BIOTRANSFORMATION 37.3(2019):190.
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