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Genome mining and metabolic profiling illuminate the chemistry driving diverse biological activities of Bacillus siamensis SCSIO 05746
Pan, Huaqi1; Tian, Xinpeng1; Shao, Mingwei1; Xie, Yunchang1; Huang, Hongbo1; Hu, Jiangchun; Ju, Jianhua1
2019
Source PublicationAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
Volume103Issue:10Pages:4153
AbstractBacillus spp. are important producers of bioactive natural products with potential applications in medicine and agriculture. Bacillus sp. SCSIO 05476 from a deep-sea sediment exhibits broad-spectrum antimicrobial activities and strong cytotoxic activity. Here, an integrative approach combining genome mining and metabolic profiling has been applied to decipher the chemical origins of this strain's varied and significant biological activities. First, genome mining revealed 19 candidate gene clusters encoding the biosynthesis of diverse secondary metabolites. Then, a series of bacillibactins, fengycins, bacillomycins, surfactins, bacillaenes, macrolactins, and related species were found by LC-DAD-MS. Finally, three new linear bacillibactins, linbacillibactins A-C (1-3), along with 11 known secondary metabolites, bacillibactin (4), normal-C-13 Val(7) surfactin (5), anteiso-C-13 Leu(7) surfactin (6), iso-C-14 Leu(7) surfactin (7), normal-C-14 Leu(7) surfactin (8), anteiso-C-14 Leu(7) surfactin (9), macrolactin D (10), normal-C-14 bacillomycin D (11), iso-C-16 bacillomycin D (12), normal-C-17 bacillomycin D (13), and iso-C-17 bacillomycin D (14), were obtained and elucidated by bioactivity and structure-guided isolation from the fermentation of strain SCSIO 05746. Among them, new compounds 1-3 show significant siderophore activities comparable to that of bacillibactin (4), compounds 13 and 14 exhibit strong cytotoxic activity. At the same time, the strain classification status was confirmed by genomic analyses, and the complete genome sequence of Bacillus siamensis was presented firstly. This study provides a foundation for understanding the mechanisms driving SCSIO 05746's multiple bioactivities and demonstrates a successful way of discovering bioactive metabolites using a combination of genome mining and metabolic profiling methods.
DepartmentLMB
KeywordBacillus Genome Secondary metabolite Siderophore Non-ribosomal peptide
DOI10.1007/s00253-019-09759-2
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Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17856
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Liaoning, Peoples R China
2.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Pan, Huaqi,Tian, Xinpeng,Shao, Mingwei,et al. Genome mining and metabolic profiling illuminate the chemistry driving diverse biological activities of Bacillus siamensis SCSIO 05746[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2019,103(10):4153, 4165.
APA Pan, Huaqi.,Tian, Xinpeng.,Shao, Mingwei.,Xie, Yunchang.,Huang, Hongbo.,...&Ju, Jianhua.(2019).Genome mining and metabolic profiling illuminate the chemistry driving diverse biological activities of Bacillus siamensis SCSIO 05746.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,103(10),4153.
MLA Pan, Huaqi,et al."Genome mining and metabolic profiling illuminate the chemistry driving diverse biological activities of Bacillus siamensis SCSIO 05746".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 103.10(2019):4153.
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