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Impact of acetolactate synthase inactivation on 1,3-propanediol fermentation by Klebsiella pneumoniae
Zhou, Sheng1; Huang, Youhua1; Mao, Xinliang2; Li, Lili1; Guo, Chuanyu1; Gao, Yongli1; Qin, Qiwei1
2019
Source PublicationPLOS ONE
ISSN1932-6203
Volume14Issue:4Pages:e0200978
Abstract1,3-Propanediol (1,3-PDO) is an important compound that is mainly used in industry for polymer production. Fermentation of 1,3-PDO from glycerol by Klebsiella pneumoniae is accompanied by formation of 2,3-butanediol (2,3-BDO) as one of the main byproduct. The first step in the formation of 2,3-BDO from pyruvate is catalyzed by acetolactate synthase (ALS), an enzyme that competes with 1,3-PDO oxidoreductase for the cofactor NADH. This study aimed to analyze the impact of engineering the 2,3-BDO formation pathway via inactivation of ALS on 1,3-PDO fermentation by K. pneumoniae HSL4. An ALS mutant was generated using Red recombinase assisted gene replacement. The ALS specific activities of K. pneumoniae Delta ALS were notably lower than that of the wild-type strain. Fed-batch fermentation of the mutant strain resulted in a 1,3-PDO concentration, productivity and conversion of 72.04 g L-1, 2.25 g L-1 h(-1), and 0.41 g g(-1), increase by 4.71%, 4.65% and 1.99% compared with the parent strain. Moreover, inactivation of ALS decreased meso-2,3-BDO formation to trace amounts, significantly increased 2S, 3S-BDO and lactate production, and a pronounced redistribution of intracellular metabolic flux was apparent.
DepartmentLMB
DOI10.1371/journal.pone.0200978
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17857
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.South China Agr Univ, Coll Marine Sci, Guangzhou, Guangdong, Peoples R China
2.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China
3.South China Univ Technol, Coll Light Ind & Food Sci, Tianhe Area, Guangzhou, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Sheng,Huang, Youhua,Mao, Xinliang,et al. Impact of acetolactate synthase inactivation on 1,3-propanediol fermentation by Klebsiella pneumoniae[J]. PLOS ONE,2019,14(4):e0200978.
APA Zhou, Sheng.,Huang, Youhua.,Mao, Xinliang.,Li, Lili.,Guo, Chuanyu.,...&Qin, Qiwei.(2019).Impact of acetolactate synthase inactivation on 1,3-propanediol fermentation by Klebsiella pneumoniae.PLOS ONE,14(4),e0200978.
MLA Zhou, Sheng,et al."Impact of acetolactate synthase inactivation on 1,3-propanediol fermentation by Klebsiella pneumoniae".PLOS ONE 14.4(2019):e0200978.
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