SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
Characterization of MtdV as a chorismate lyase essential to A201A biosynthesis and precursor-directed biosynthesis of new analogs
Zhu, Qinghua1; Song, Yongxiang; Huang, Hongbo; Li, Qinglian; Ju, Jianhua2
2019
Source PublicationORGANIC & BIOMOLECULAR CHEMISTRY
ISSN1477-0520
Volume17Issue:15Pages:3760
AbstractThe antimicrobial nucleoside antibiotic A201A is produced by the deep-sea derived Marinactinospora thermotolerans SCSIO 00652. Bioinformatics analysis revealed that mtdV, downstream within the A201A biosynthetic gene cluster, encodes a protein with low homology to a group of chorismate pyruvatelyases. To explore the role of mtdV in A201A biosynthesis, mtdV was inactivated and HPLC analysis revealed that the resulting Delta mtdV mutant failed to produce A201A; production was partially restored by adding exogenous 4-hydroxybenzoic acid (4HB) to the fermentation. In vitro biochemical assays showed that MtdV catalyzes the conversion of chorismate into 4HB, thereby firmly demonstrating that MtdV is a chorismate lyase involved in A201A biosynthesis. In addition, supplementation of the Delta mtdV mutant with various 4HB analogs enabled production of seven new A201A analogs. Antimicrobial assays showed that the purified A201A analogs 3'-F-A201A and 3'-Cl-A201A were just as active as A201A against the test strains with MIC values of 1-8 mu g mL(-1).
DepartmentLMB
DOI10.1039/c8ob02852d
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17858
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China
2.Dezhou Univ, Key Lab Biotechnol & Biol Resource Utilizat Univ, Dezhou 253023, Peoples R China
3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 10049, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Qinghua,Song, Yongxiang,Huang, Hongbo,et al. Characterization of MtdV as a chorismate lyase essential to A201A biosynthesis and precursor-directed biosynthesis of new analogs[J]. ORGANIC & BIOMOLECULAR CHEMISTRY,2019,17(15):3760, 3764.
APA Zhu, Qinghua,Song, Yongxiang,Huang, Hongbo,Li, Qinglian,&Ju, Jianhua.(2019).Characterization of MtdV as a chorismate lyase essential to A201A biosynthesis and precursor-directed biosynthesis of new analogs.ORGANIC & BIOMOLECULAR CHEMISTRY,17(15),3760.
MLA Zhu, Qinghua,et al."Characterization of MtdV as a chorismate lyase essential to A201A biosynthesis and precursor-directed biosynthesis of new analogs".ORGANIC & BIOMOLECULAR CHEMISTRY 17.15(2019):3760.
Files in This Item:
File Name/Size DocType Version Access License
Characterization of (1358KB)期刊论文出版稿开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.