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HRI of Epinephelus coioides is a critical factor in the grouper immune response to RGNNV infection
Zang, Shaoqing1,4; Zhang, Xin2; Li, Chen2; Wang, Liqun1,4; Wei, Jingguang2,5; Qin, Qiwei1,2,3,4
2019
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume87Pages:659
AbstractPhosphorylation of eukaryotic initiation factor 2 alpha subunit (eIF2 alpha) occurs under a variety of conditions, including viral infection. Heme-regulated inhibitor (HRI) is an eIF2 alpha kinase that modifies this phosphorylation. In this study, a HRI homologue (EcHRI) from the orange-spotted grouper (Epinephelus coioides) was cloned and its roles during fish viral infection were characterized. EcHRI encodes a 664-amino acid polypeptide that shares a high degree of similarity with HRIs from other species. Quantitative real-time polymerase chain reaction analysis indicated that EcHRI was distributed in all examined tissues. Expression of EcHRI in the spleen of E. coioides was up-regulated when challenged with the synthetic analog of double-stranded RNA (dsRNA) of polyinosine-polycytidylic acid (poly I:C). EcHRI was significantly increased in red-spotted grouper nervous necrosis virus (RGNNV) infected cells. EcHRI was abundantly distributed in the nucleus of grouper spleen (GS) cells. Overexpression of EcHRI inhibited the expression of red-spotted grouper nervous necrosis virus (RGNNV) genes in GS cells. Furthermore, our results showed that EcHRI overexpression significantly increased the expression of interferon (IFN)-related cytokines and enhanced activation of IFN-beta, interferon-sensitive response element (ISRE), and nuclear factor kappa B (NF-kappa B). Taken together, these results suggest that EcHRI is involved in the fish immune response to virus challenge.
DepartmentLMB
KeywordHRI eIF2 alpha kinase Epinephelus coioides RGNNV
DOI10.1016/j.fsi.2019.02.011
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17871
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Shandong, Peoples R China
5.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China
6.Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou 510642, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Zang, Shaoqing,Zhang, Xin,Li, Chen,et al. HRI of Epinephelus coioides is a critical factor in the grouper immune response to RGNNV infection[J]. FISH & SHELLFISH IMMUNOLOGY,2019,87:659, 668.
APA Zang, Shaoqing,Zhang, Xin,Li, Chen,Wang, Liqun,Wei, Jingguang,&Qin, Qiwei.(2019).HRI of Epinephelus coioides is a critical factor in the grouper immune response to RGNNV infection.FISH & SHELLFISH IMMUNOLOGY,87,659.
MLA Zang, Shaoqing,et al."HRI of Epinephelus coioides is a critical factor in the grouper immune response to RGNNV infection".FISH & SHELLFISH IMMUNOLOGY 87(2019):659.
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