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Cytochrome P450 oxidase SlgO1 catalyzes the biotransformation of tirandamycin C to a new tirandamycin derivative
Mo, Xuhua; Gui, Chun1; Yang, Song
2019
Source Publication3 BIOTECH
ISSN2190-572X
Volume9Issue:3Pages:71
AbstractIn the present study, an Escherichia coli whole cell system with overexpression of a cytochrome P450 oxidase SlgO1 involved in streptolydigin biosynthetic pathway, an E. coli flavodoxin NADP(+) oxidoreductase (EcFLDR), and an E. coli flavodoxin A (EcFLDA) were constructed. Biotransformation experiments revealed that SlgO1 can convert tirandamycin C to tirandamycin F, indicating that it can introduce a hydroxyl group into the C-10 position of tirandamycin C. Subsequently, slgO1 was cloned into pSET152AKE vector under the downstream of ermE* promoter, which was, respectively, introduced into Streptomyces sp. SCSIO1666 (tirandamycin B producer), Streptomyces sp. Ju1008 (tirandamycin C producer), and Streptomyces sp. Ju1009 (tirandamycin E producer). A novel tirandamycin derivative tirandamycin L accumulated in the engineered strain Streptomyces sp. Ju1008::slgO1 was isolated and its structure was determined on the basis of nuclear magnetic resonance (NMR) and mass spectrometry. Unlike most of the identified tirandamycins, tirandamycin L possessed a rare C-11-C-12 saturated bond as well as a C-10 ketone moiety. In addition, tirandamycin L showed weaker antibacterial activity. Based on the structure of tirandamycin L, SlgO1 was proposed to be responsible for multiple modifications toward tirandamycin C, including the formation of C-10 hydroxyl and C-11-C-12 saturated bond.
DepartmentLMB
KeywordTirandamycins Streptolydigin Cytochrome P450 oxidase SlgO1 Combinatorial biosynthesis
DOI10.1007/s13205-019-1611-1
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17889
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Qingdao Agr Univ, Shandong Prov Key Lab Appl Mycol, Sch Life Sci, Qingdao 266109, Peoples R China
2.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Mo, Xuhua,Gui, Chun,Yang, Song. Cytochrome P450 oxidase SlgO1 catalyzes the biotransformation of tirandamycin C to a new tirandamycin derivative[J]. 3 BIOTECH,2019,9(3):71.
APA Mo, Xuhua,Gui, Chun,&Yang, Song.(2019).Cytochrome P450 oxidase SlgO1 catalyzes the biotransformation of tirandamycin C to a new tirandamycin derivative.3 BIOTECH,9(3),71.
MLA Mo, Xuhua,et al."Cytochrome P450 oxidase SlgO1 catalyzes the biotransformation of tirandamycin C to a new tirandamycin derivative".3 BIOTECH 9.3(2019):71.
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