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Uptake and subcellular distribution of aluminum in a marine diatom
Liu, Qingxia2,3; Zhou, Linbin2,3; Liu, Fengjie; Fortin, Claude; Tan, Yehui2; Huang, Liangmin2,3; Campbell, Peter G. C.
AbstractAluminum (Al) is widespread in the environment including the ocean. The effects of Al on marine organisms have attracted more and more attention in recent years. However, the mechanisms of uptake of Al by marine organisms and the subcellular distribution of Al once assimilated are unknown. Here we report the uptake and subcellular distribution of Al in a marine diatom Thalassiosira weissflogii. Short-term (< 120 min) uptake experiments showed that the Al uptake rate by the diatom was 0.033 +/- 0.013 fmol(-1) cell(-1) min(-1) (internalization flux normalized to the exposure Al concentration of 2 mu M = 0.034 +/- 0.013 nmol m(-2) min(-1) nN(-1)). Subcellular fractionation experiments showed that the internalized Al was partitioned to subcellular components in the following order: granules (69 +/- 5%) > debris (17 +/- 4%) > organelles (12 +/- 2%) > heat-stable peptides (HSP) (similar to 2%) > heat-denaturable proteins (HDP) (< 1%), indicating that the majority of intracellular Al was detoxified and stored in inorganic forms. The subcellular distribution of Al in the diatom is different from that of Al in freshwater green algae, in which most of the internalized Al is partitioned to organelles. We also evaluated an artificial seawater-based EDTA rinse solution to remove Al adsorbed on the diatom cell surface. Overall, our study provides new information to understand the mechanisms of uptake of Al by marine diatoms, and the mechanisms responsible for the biological effects (both toxic and beneficial) of Al on the growth of marine phytoplankton, especially diatoms.
KeywordAluminum Uptake Subcellular distribution Marine diatom Thalassiosira weissfiogii
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China
2.Zhou, Linbin; Liu, Fengjie; Fortin, Claude; Campbell, Peter G. C.] Ctr Eau Terre Environm, Inst Natl Rech Sci, Quebec City, PQ, Canada
3.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou, Guangdong, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Liu, Qingxia,Zhou, Linbin,Liu, Fengjie,et al. Uptake and subcellular distribution of aluminum in a marine diatom[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2019,169:85, 92.
APA Liu, Qingxia.,Zhou, Linbin.,Liu, Fengjie.,Fortin, Claude.,Tan, Yehui.,...&Campbell, Peter G. C..(2019).Uptake and subcellular distribution of aluminum in a marine diatom.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,169,85.
MLA Liu, Qingxia,et al."Uptake and subcellular distribution of aluminum in a marine diatom".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 169(2019):85.
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