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Functional characterization of the halogenase SpmH and discovery of new deschloro-tryptophan dimers
Liu, Zhiwen; Ma, Liang; Zhang, Liping; Zhang, Wenjun; Zhu, Yiguang; Chen, Yuchan1; Zhang, Weimin1; Zhang, Changsheng
2019
Source PublicationORGANIC & BIOMOLECULAR CHEMISTRY
ISSN1477-0520
Volume17Issue:5Pages:1053
AbstractThe halogenase gene spmH was putatively involved in the biosynthesis of spiroindimicins/indimicins (SPMs/IDMs), a group of chlorinated tryptophan dimers (TDs) from deep-sea-derived Streptomyces sp. SCSIO 03032. Inactivation of spmH led to six deschloro-analogues of TDs, including four new compounds SPMs G (1) and H (2), and IDMs F (3) and G (4). The structures and absolute configurations of 1-4 were unambiguously determined by the combination of extensive spectroscopic analysis, single-crystal X-ray diffraction and quantum chemical ECD calculations. Compounds 1 and 2 exhibited moderate cytotoxic activities against four cancer cell lines. Additionally, SpmH was biochemically characterized in vitro as an L-tryptophan 5-halogenase.
DepartmentLMB
DOI10.1039/c8ob02775g
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17899
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Chinese Acad Sci, Guangdong Key Lab Marine Mat Med, Key Lab Trop Marine Bioresources & Ecol, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China
2.Guangdong Inst Microbiol, State Key Lab Appl Microbiol Southern China, 100 Cent Xianlie Rd, Guangzhou 510070, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Liu, Zhiwen,Ma, Liang,Zhang, Liping,et al. Functional characterization of the halogenase SpmH and discovery of new deschloro-tryptophan dimers[J]. ORGANIC & BIOMOLECULAR CHEMISTRY,2019,17(5):1053, 1057.
APA Liu, Zhiwen.,Ma, Liang.,Zhang, Liping.,Zhang, Wenjun.,Zhu, Yiguang.,...&Zhang, Changsheng.(2019).Functional characterization of the halogenase SpmH and discovery of new deschloro-tryptophan dimers.ORGANIC & BIOMOLECULAR CHEMISTRY,17(5),1053.
MLA Liu, Zhiwen,et al."Functional characterization of the halogenase SpmH and discovery of new deschloro-tryptophan dimers".ORGANIC & BIOMOLECULAR CHEMISTRY 17.5(2019):1053.
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