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Identification of the Anti-Infective Aborycin Biosynthetic Gene Cluster from Deep-Sea-Derived Streptomyces sp. SCSIO ZS0098 Enables Production in a Heterologous Host
Shao, Mingwei1; Ma, Juying1; Li, Qinglian1; Ju, Jianhua1
2019
Source PublicationMARINE DRUGS
ISSN1660-3397
Volume17Issue:2Pages:127
AbstractAborycin is a ribosomally synthesized member of the type I lasso peptide natural products. In the present study, aborycin was isolated and identified from the deep-sea-derived microbe Streptomyces sp. SCSIO ZS0098. The aborycin biosynthetic gene cluster (abo) was identified on the basis of genome sequence analyses and then heterologously expressed in Streptomyces coelicolor M1152 to effectively produce aborycin. Aborycin generated in this fashion exhibited moderate antibacterial activity against 13 Staphylococcus aureus strains from various sources with minimum inhibitory concentrations MICs = 8.0 128 mu g/mL, against Enterococcus faecalis ATCC 29212 with an MIC = 8.0 mu g/mL, and against Bacillus thuringiensis with MIC = 2.0 mu g/mL. Additionally, aborycin displayed potent antibacterial activity (MIC = 0.5 mu g/mL) against the poultry pathogen Enterococcus gallinarum 5F52C. The reported abo cluster clearly has the potential to provide a means of expanding the repertoire of anti-infective type I lasso peptides.
DepartmentLMB
KeywordStreptomyces sp SCSIO ZS0098 aborycin heterogenous expression antibacterial activity
DOI10.3390/md17020127
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17905
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, South China Sea Inst Oceanol,RNAM Ctr Marine Micr, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China
2.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 110039, Peoples R China
Recommended Citation
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Shao, Mingwei,Ma, Juying,Li, Qinglian,et al. Identification of the Anti-Infective Aborycin Biosynthetic Gene Cluster from Deep-Sea-Derived Streptomyces sp. SCSIO ZS0098 Enables Production in a Heterologous Host[J]. MARINE DRUGS,2019,17(2):127.
APA Shao, Mingwei,Ma, Juying,Li, Qinglian,&Ju, Jianhua.(2019).Identification of the Anti-Infective Aborycin Biosynthetic Gene Cluster from Deep-Sea-Derived Streptomyces sp. SCSIO ZS0098 Enables Production in a Heterologous Host.MARINE DRUGS,17(2),127.
MLA Shao, Mingwei,et al."Identification of the Anti-Infective Aborycin Biosynthetic Gene Cluster from Deep-Sea-Derived Streptomyces sp. SCSIO ZS0098 Enables Production in a Heterologous Host".MARINE DRUGS 17.2(2019):127.
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