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Excisionase in Pf filamentous prophage controls lysis-lysogeny decision-making in Pseudomonas aeruginosa
Li, Yangmei1; Liu, Xiaoxiao; Tang, Kaihao; Wang, Pengxia; Zeng, Zhenshun; Guo, Yunxue; Wang, Xiaoxue1
2019
Source PublicationMOLECULAR MICROBIOLOGY
ISSN0950-382X
Volume111Issue:2Pages:495
AbstractPf filamentous prophages are prevalent among clinical and environmental Pseudomonas aeruginosa isolates. Pf4 and Pf5 prophages are integrated into the host genomes of PAO1 and PA14, respectively, and play an important role in biofilm development. However, the genetic factors that directly control the lysis-lysogeny switch in Pf prophages remain unclear. Here, we identified and characterized the excisionase genes in Pf4 and Pf5 (named xisF4 and xisF5, respectively). XisF4 and XisF5 represent two major subfamilies of functional excisionases and are commonly found in Pf prophages. While both of them can significantly promote prophage excision, only XisF5 is essential for Pf5 excision. XisF4 activates Pf4 phage replication by upregulating the phage initiator gene (PA0727). In addition, xisF4 and the neighboring phage repressor c gene pf4r are transcribed divergently and their 5 '-untranslated regions overlap. XisF4 and Pf4r not only auto-activate their own expression but also repress each other. Furthermore, two H-NS family proteins, MvaT and MvaU, coordinately repress Pf4 production by directly repressing xisF4. Collectively, we reveal that Pf prophage excisionases cooperate in controlling lysogeny and phage production.
DepartmentLMB
DOI10.1111/mmi.14170
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17913
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, Guangzhou 510301, Guangdong, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Li, Yangmei,Liu, Xiaoxiao,Tang, Kaihao,et al. Excisionase in Pf filamentous prophage controls lysis-lysogeny decision-making in Pseudomonas aeruginosa[J]. MOLECULAR MICROBIOLOGY,2019,111(2):495, 513.
APA Li, Yangmei.,Liu, Xiaoxiao.,Tang, Kaihao.,Wang, Pengxia.,Zeng, Zhenshun.,...&Wang, Xiaoxue.(2019).Excisionase in Pf filamentous prophage controls lysis-lysogeny decision-making in Pseudomonas aeruginosa.MOLECULAR MICROBIOLOGY,111(2),495.
MLA Li, Yangmei,et al."Excisionase in Pf filamentous prophage controls lysis-lysogeny decision-making in Pseudomonas aeruginosa".MOLECULAR MICROBIOLOGY 111.2(2019):495.
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