SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
Characterization of Bacillus subtilis from gastrointestinal tract of hybrid Hulong grouper (Epinephelus fuscoguttatus x E-lanceolatus) and its effects as probiotic additives
Zhou, Sheng; Song, Deli2; Zhou, Xiaofeng; Mao, Xinliang; Zhou, Xuefeng3; Wang, Sunli; Wei, Jingguang; Huang, Youhua; Wang, Wenxiong4; Xiao, Su-Mei; Qin, Qiwei
2019
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume84Pages:1115
AbstractProbiotics are widely used for the improvement of animals' growth and health. However, few marine aquatic probiotics are applied and licensed in China. In this study, a Bacillus spp. strain was isolated from the Hulong grouper gastrointestinal tract, which was identified as a new strain of Bacillus subtilis and was named as 7k. B. subtilis 7k showed desirable capability of sporulation and resistance to heat, simulated gastric juice and simulated duodenum juice, indicating its potential as probiotics. Seven antimicrobial chemicals were found in the secretion of the B. subtilis 7k. B. subtilis 7k addition in diet promoted the growth rate of Hulong groupers. Moreover, B. subtilis 7k can inhibit infection by iridovirus, making B. subtilis 7k a suitable kind of probiotic for maintaining fishes' health. Our results also revealed that B. subtilis 7k induced non-specific immune response in Hulong grouper under virus infection. Hulong grouper fed by diets containing B. subtilis 7k at 10(8) and 10(10) cfu g(-1) for 4-8 weeks were significantly strengthened in serum lysozyme activity, serum alternative complement activity (ACH50), serum bactericidal activity, respiratory burst, superoxide dismutase activity (SOD), and phagocytic activity of head kidney leucocytes when compared with those fed by control diets. In conclusion, B. subtilis 7k was isolated and characterized to be a kind of process enduring, growth stimulating, immunity enhancing and health promoting probiotic using in grouper culture.
DepartmentLMB
KeywordBacillus subtilis Probiotic Grouper Growth Innate immunity
DOI10.1016/j.fsi.2018.10.058
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17924
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.South China Agr Univ, Coll Marine Sci, Joint Lab Guangdong Prov & Hong Kong Reg Marine B, Guangzhou 510642, Guangdong, Peoples R China
2.Sun Yat Sen Univ, Sch Publ Hlth, Dept Med Stat & Epidemiol, Guangzhou 510080, Guangdong, Peoples R China
3.Sun Yat Sen Univ, Zhongshan Sch Med, Dept Pharmacol, Guangzhou 510080, Guangdong, Peoples R China
4.Chinese Acad Sci, South China Sea Inst Oceanol, Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
5.Hong Kong Univ Sci & Technol, Dept Ocean Sci, Kowloon, Clearwater Bay, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Sheng,Song, Deli,Zhou, Xiaofeng,et al. Characterization of Bacillus subtilis from gastrointestinal tract of hybrid Hulong grouper (Epinephelus fuscoguttatus x E-lanceolatus) and its effects as probiotic additives[J]. FISH & SHELLFISH IMMUNOLOGY,2019,84:1115, 1124.
APA Zhou, Sheng.,Song, Deli.,Zhou, Xiaofeng.,Mao, Xinliang.,Zhou, Xuefeng.,...&Qin, Qiwei.(2019).Characterization of Bacillus subtilis from gastrointestinal tract of hybrid Hulong grouper (Epinephelus fuscoguttatus x E-lanceolatus) and its effects as probiotic additives.FISH & SHELLFISH IMMUNOLOGY,84,1115.
MLA Zhou, Sheng,et al."Characterization of Bacillus subtilis from gastrointestinal tract of hybrid Hulong grouper (Epinephelus fuscoguttatus x E-lanceolatus) and its effects as probiotic additives".FISH & SHELLFISH IMMUNOLOGY 84(2019):1115.
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