SCSIO OpenIR  > 中科院边缘海地质重点实验室
Quantitative proteomics reveals the key molecular events occurring at different cell cycle phases of the in situ blooming dinoflagellate cells
Zhang, Hao; Liu, Jiuling1; He, Yanbin2; Xie, Zhangxian; Zhang, Shufei3; Zhang, Yong; Lin, Lin; Liu, Siqi2; Wang, Dazhi
2019
Source PublicationSCIENCE OF THE TOTAL ENVIRONMENT
ISSN0048-9697
Volume676Pages:62
AbstractDinoflagellate blooms are the results of rapid cell proliferation governed by cell cycle, a highly-ordered series of events that culminates in cell division. However, little is known about cell cycle progression of the in situ bloom cells. Here, we compared proteomes of the in situ blooming cells of a dinoflagellate Prorocentrum donghaiense collected at different cell cycle phases. The blooming P. donghaiense cells completed a cell cycle within 24 h with a high synchronization rate of 82.7%. Proteins associated with photosynthesis, porphyrin and chlorophyll synthesis, carbon, nitrogen and amino add metabolisms exhibited high expressions at the G(1) phase; DNA replication and mismatch repair related proteins were more abundant at the S phase; while protein synthesis and oxidative phosphorylation were highly enriched at the G(2)/M phase. Cell cycle proteins presented similar periodic did patterns to other eukaryotic cells, and higher expressions of proliferating cell nuclear antigen and cyclin dependent kinase 2 at the S phase ensured the smooth S-G(2)/M transition. Strikingly, four histones were first identified in P. donghaiense and highly expressed at the G(2)/M phase, indicating their potential roles in regulating cell cycle. This study presents the first quantitative survey, to our knowledge, of proteome changes at different cell cycle phases of the in situ blooming cells in natural environment and provides insights into cell cycle regulation of the blooming dinoflagellate cells. (C) 2019 Elsevier B.V. All rights reserved.
DepartmentOMG
KeywordCell cycle Dinoflagellates Harmful algal blooms iTRAQ Quantitative proteomics
DOI10.1016/j.scitotenv.2019.04.216
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17944
Collection中科院边缘海地质重点实验室
Affiliation1.Xiamen Univ, State Key Lab Marine Environm Sci, Coll Environm & Ecol, Xiamen 361005, Peoples R China
2.Chinese Acad Sci, Key Lab Ocean & Marginal Sea Geol, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
3.BGI Shenzhen, Beishan Ind Zone 11th Bldg, Shenzhen 518083, Guangdong, Peoples R China
4.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Hao,Liu, Jiuling,He, Yanbin,et al. Quantitative proteomics reveals the key molecular events occurring at different cell cycle phases of the in situ blooming dinoflagellate cells[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2019,676:62, 71.
APA Zhang, Hao.,Liu, Jiuling.,He, Yanbin.,Xie, Zhangxian.,Zhang, Shufei.,...&Wang, Dazhi.(2019).Quantitative proteomics reveals the key molecular events occurring at different cell cycle phases of the in situ blooming dinoflagellate cells.SCIENCE OF THE TOTAL ENVIRONMENT,676,62.
MLA Zhang, Hao,et al."Quantitative proteomics reveals the key molecular events occurring at different cell cycle phases of the in situ blooming dinoflagellate cells".SCIENCE OF THE TOTAL ENVIRONMENT 676(2019):62.
Files in This Item:
File Name/Size DocType Version Access License
Quantitative proteom(2151KB)期刊论文出版稿开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.