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An Approach for In Situ Rapid Detection of Deep-Sea Aromatic Amino Acids Using Laser-Induced Fluorescence
Du, Ranran1; Yang, Dingtian2; Jiang, Guangjia3; Song, Youren; Yin, Xiaoqing1
2020
Source PublicationSENSORS
Volume20Issue:5Pages:1330
AbstractAmino acids are the material basis of almost all life activities. An improved understanding of the source, state, and cycle of amino acids is essential for determining the energy flow and material circulation of marine ecosystems. In the present study, an in situ rapid detection method of ultraviolet (UV; 266 nm) laser-induced fluorescence (LIF) technology was used to detect three natural, aromatic amino acids in the seawater. The laser-induced fluorescence peaks of aromatic amino acids tryptophan, tyrosine, and phenylalanine were located at 350 nm, 300 nm, and 280 nm, respectively. High, linear correlations between the concentrations of the aromatic amino acids and the fluorescence peak heights were observed, and the lowest detectable concentrations of tryptophan, tyrosine, and phenylalanine were 4.70 x 10(-9) mol/L, 2.76 x 10(-8) mol/L, and 6.05 x 10(-7) mol/L, respectively, which allowed us to quantify their concentrations by using laser-induced fluorescence. This paper not only provides a practical method for the detection of aromatic amino acids in seawater, but a new means to further understand the biogeochemical processes of carbon cycles in the deep sea.
DepartmentLTO
Keywordsoluble aromatic amino acid laser-induced fluorescence in situ detection
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/18340
Collection热带海洋环境国家重点实验室(LTO)
Affiliation1.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Ocean Remote Sensing, State Key Lab Trop Oceanog, Guangzhou 510301, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Southern Marine Sci & Engn Guangdong Lab, Guangzhou 511458, Peoples R China
4.State Ocean Adm, South China Sea Environm Monitoring Ctr, Guangzhou 510300, Peoples R China
Recommended Citation
GB/T 7714
Du, Ranran,Yang, Dingtian,Jiang, Guangjia,et al. An Approach for In Situ Rapid Detection of Deep-Sea Aromatic Amino Acids Using Laser-Induced Fluorescence[J]. SENSORS,2020,20(5):1330.
APA Du, Ranran,Yang, Dingtian,Jiang, Guangjia,Song, Youren,&Yin, Xiaoqing.(2020).An Approach for In Situ Rapid Detection of Deep-Sea Aromatic Amino Acids Using Laser-Induced Fluorescence.SENSORS,20(5),1330.
MLA Du, Ranran,et al."An Approach for In Situ Rapid Detection of Deep-Sea Aromatic Amino Acids Using Laser-Induced Fluorescence".SENSORS 20.5(2020):1330.
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