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Type III Secretion 1 Effector Gene Diversity AmongVibrioIsolates From Coastal Areas in China
Wu, Chao; Zhao, Zhe; Liu, Yupeng; Zhu, Xinyuan; Liu, Min; Luo, Peng1; Shi, Yan
2020
Source PublicationFRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
ISSN2235-2988
Volume10Pages:301
AbstractVibrios, which include more than 120 valid species, are an abundant and diverse group of bacteria in marine and estuarine environments. Some of these bacteria have been recognized as pathogens of both marine animals and humans, and therefore, their virulence mechanisms have attracted increasing attention. The type III secretion system (T3SS) is an important virulence determinant in many gram-negative bacteria, in which this system directly translocates variable effectors into the host cytosol for the manipulation of the cellular responses. In this study, the distribution of the T3SS gene cluster was first examined in 110Vibriostrains of 26 different species, including 98 strains isolated from coastal areas in China. Several T3SS1 genes, but not T3SS2 genes (T3SS2 alpha and T3SS2 beta), were universally detected in all the strains of four species,Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio harveyi, andVibrio campbellii. The effector coding regions within the T3SS1 gene clusters from the T3SS1-positive strains were further analyzed, revealing that variations in the effectors ofVibrioT3SS1 were observed among the fourVibriospecies, even between different strains inV. harveyi, according to their genetic organization. Importantly, Afp17, a potential novel effector that may exert a similar function as the known effector VopS in T3SS1-induced cell death, based on cytotoxicity assay results, was found in the effector coding region of the T3SS1 in someV. harveyiandV. campbelliistrains. Finally, it was revealed that differences in T3SS1-mediated cytotoxicity were dependent not only on the variations in the effectors ofVibrioT3SS1 but also on the initial adhesion ability to host cells, which is another prerequisite condition. Altogether, our results contribute to the clarification of the diversity of T3SS1 effectors and a better understanding of the differences in cytotoxicity amongVibriospecies.
DepartmentLMB
KeywordVibrios type III secretion system (T3SS) cytotoxicity effector bacterial adhesion
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31872597]; Natural Science Foundation of Jiangsu ProvinceJiangsu Planned Projects for Postdoctoral Research FundsNatural Science Foundation of Jiangsu Province [BK20171431]; Jiangsu Agriculture Science and Technology Innovation Fund [CX [19]2033]; Earmarked Fund for Jiangsu Agricultural Industry Technology System [JATS [2019]477] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31872597]; Natural Science Foundation of Jiangsu ProvinceJiangsu Planned Projects for Postdoctoral Research FundsNatural Science Foundation of Jiangsu Province [BK20171431]; Jiangsu Agriculture Science and Technology Innovation Fund [CX [19]2033]; Earmarked Fund for Jiangsu Agricultural Industry Technology System [JATS [2019]477]
DOI10.3389/fcimb.2020.00301
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31872597]; Natural Science Foundation of Jiangsu ProvinceJiangsu Planned Projects for Postdoctoral Research FundsNatural Science Foundation of Jiangsu Province [BK20171431]; Jiangsu Agriculture Science and Technology Innovation Fund [CX [19]2033]; Earmarked Fund for Jiangsu Agricultural Industry Technology System [JATS [2019]477] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31872597]; Natural Science Foundation of Jiangsu ProvinceJiangsu Planned Projects for Postdoctoral Research FundsNatural Science Foundation of Jiangsu Province [BK20171431]; Jiangsu Agriculture Science and Technology Innovation Fund [CX [19]2033]; Earmarked Fund for Jiangsu Agricultural Industry Technology System [JATS [2019]477]
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Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/18394
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Hohai Univ, Coll Oceanog, Dept Marine Biol, Nanjing, Peoples R China
2.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou, Peoples R China
Recommended Citation
GB/T 7714
Wu, Chao,Zhao, Zhe,Liu, Yupeng,et al. Type III Secretion 1 Effector Gene Diversity AmongVibrioIsolates From Coastal Areas in China[J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2020,10:301.
APA Wu, Chao.,Zhao, Zhe.,Liu, Yupeng.,Zhu, Xinyuan.,Liu, Min.,...&Shi, Yan.(2020).Type III Secretion 1 Effector Gene Diversity AmongVibrioIsolates From Coastal Areas in China.FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,10,301.
MLA Wu, Chao,et al."Type III Secretion 1 Effector Gene Diversity AmongVibrioIsolates From Coastal Areas in China".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 10(2020):301.
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