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Transcriptomic analysis of sea cucumber (Holothuria leucospilota) coelomocytes revealed the echinoderm cytokine response during immune challenge
Wu, Xiaofen1; Chen, Ting2; Huo, Da2,3; Yu, Zonghe2,3; Ruan, Yao1; Cheng, Chuhang1; Jiang, Xiao2,3; Ren, Chunhua2,3
2020
Source PublicationBMC GENOMICS
ISSN1471-2164
Volume21Issue:1
AbstractBackgroundThe sea cucumber Holothuria leucospilota belongs to echinoderm, which is evolutionally the most primitive group of deuterostomes. Sea cucumber has a cavity between its digestive tract and the body wall that is filled with fluid and suspended coelomic cells similar to blood cells. The humoral immune response of the sea cucumber is based on the secretion of various immune factors from coelomocytes into the coelomic cavity. The aim of this study is to lay out a foundation for the immune mechanisms in echinoderms and their origins in chordates by using RNA-seq.ResultsSea cucumber primary coelomocytes were isolated from healthy H. leucospilota and incubated with lipopolysaccharide (LPS, 10 mu g/ml), polyinosinic-polycytidylic acid [Poly (I:C), 10 mu g/ml] and heat-inactived Vibrio harveyi (10(7) cell/ml) for 24h, respectively. After high-throughput mRNA sequencing on an Illumina HiSeq2500, a de novo transcriptome was assembled and the Unigenes were annotated. Thirteen differentially expressed genes (DEGs) were selected randomly from our data and subsequently verified by using RT-qPCR. The results of RT-qPCR were consistent with those of the RNA-seq (R-2=0.61). The top 10 significantly enriched signaling pathways and immune-related pathways of the common and unique DEGs were screened from the transcriptome data. Twenty-one cytokine candidate DEGs were identified, which belong to 4 cytokine families, namely, BCL/CLL, EPRF1, IL-17 and TSP/TPO. Gene expression in response to LPS dose-increased treatment (0, 10, 20 and 50 mu g/ml) showed that IL-17 family cytokines were significantly upregulated after 10 mu g/ml LPS challenge for 24h.ConclusionA de novo transcriptome was sequenced and assembled to generate the gene expression profiling across the sea cucumber coelomocytes treated with LPS, Poly (I:C) and V. harveyi. The cytokine genes identified in DEGs could be classified into 4 cytokine families, in which the expression of IL-17 family cytokines was most significantly induced after 10 mu g/ml LPS challenge for 24h. Our findings have laid the foundation not only for the research of molecular mechanisms related to the immune response in echinoderms but also for their origins in chordates, particularly in higher vertebrates.
DepartmentLMB
KeywordSea cucumber Coelomocytes Transcriptome RNA-seq Differentailly expressed genes Immune response Cytokines
Funding OrganizationNational Key R&D Program of China [2018YFD0901605]; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA13020205]; Science & Technology Promoting Projects for Oceanic & Fishery in Guangdong Province [SDYY-2018-01]; Guangdong Province Program [2017B030314052, 2018A030313857, 2015A030310120, A2015230]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41676162]; Science and Technology Service Network Initiative of Chinese Academy of Sciences [KFJ-STS-ZDTP-055]; Science and Technology Research Programs of Guangdong Province [2018A030313857] ; National Key R&D Program of China [2018YFD0901605]; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA13020205]; Science & Technology Promoting Projects for Oceanic & Fishery in Guangdong Province [SDYY-2018-01]; Guangdong Province Program [2017B030314052, 2018A030313857, 2015A030310120, A2015230]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41676162]; Science and Technology Service Network Initiative of Chinese Academy of Sciences [KFJ-STS-ZDTP-055]; Science and Technology Research Programs of Guangdong Province [2018A030313857]
DOI10.1186/s12864-020-6698-6
Funding OrganizationNational Key R&D Program of China [2018YFD0901605]; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA13020205]; Science & Technology Promoting Projects for Oceanic & Fishery in Guangdong Province [SDYY-2018-01]; Guangdong Province Program [2017B030314052, 2018A030313857, 2015A030310120, A2015230]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41676162]; Science and Technology Service Network Initiative of Chinese Academy of Sciences [KFJ-STS-ZDTP-055]; Science and Technology Research Programs of Guangdong Province [2018A030313857] ; National Key R&D Program of China [2018YFD0901605]; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA13020205]; Science & Technology Promoting Projects for Oceanic & Fishery in Guangdong Province [SDYY-2018-01]; Guangdong Province Program [2017B030314052, 2018A030313857, 2015A030310120, A2015230]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41676162]; Science and Technology Service Network Initiative of Chinese Academy of Sciences [KFJ-STS-ZDTP-055]; Science and Technology Research Programs of Guangdong Province [2018A030313857]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/18448
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, South China Sea Inst Oceanol, Guangzhou 510301, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst South China Sea Ecol & Environm Engn, ISEE, Guangzhou, Peoples R China
4.South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510301, Peoples R China
Recommended Citation
GB/T 7714
Wu, Xiaofen,Chen, Ting,Huo, Da,et al. Transcriptomic analysis of sea cucumber (Holothuria leucospilota) coelomocytes revealed the echinoderm cytokine response during immune challenge[J]. BMC GENOMICS,2020,21(1).
APA Wu, Xiaofen.,Chen, Ting.,Huo, Da.,Yu, Zonghe.,Ruan, Yao.,...&Ren, Chunhua.(2020).Transcriptomic analysis of sea cucumber (Holothuria leucospilota) coelomocytes revealed the echinoderm cytokine response during immune challenge.BMC GENOMICS,21(1).
MLA Wu, Xiaofen,et al."Transcriptomic analysis of sea cucumber (Holothuria leucospilota) coelomocytes revealed the echinoderm cytokine response during immune challenge".BMC GENOMICS 21.1(2020).
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