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学科主题: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical
题名: Employment of reactive oxygen species to enhance astaxanthin formation in Chlorella zofingiensis in heterotrophic culture
作者: Ip, PF ; Chen, F
通讯作者: sfchen@hkusua.hku.hk
关键词: Chlorella zofingiensis ; heterotrophic culture ; secondary carotenoids ; astaxanthin ; oxidative stress ; reactive oxygen species
刊名: PROCESS BIOCHEMISTRY
发表日期: 2005
卷: 40, 期:11, 页:3491-3496
收录类别: sci
部门归属: Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Peoples R China
项目归属: 广东省海洋药物重点实验室
摘要: The advantages of using heterotrophic cultivation for commercial production of the high-value ketocarotenoid astaxanthin by microalgae have been emphasized in recent years [Chen F. High cell density culture of microalgae in heterotrophic growth. Trends Biotechnol 1996;14:421-5; Chen F, Chen H, Gong X. Mixotrophic and heterotrophic growth of Haematococcus lacustris and rheological behaviour of the cell suspensions. Bioresour Technol 1997;62:19-24; Ip, PF, Chen F. Production of astaxanthin by the green microalga Chlorella zofingiensis in the dark. Process Biochem 2005;40:733-8]. In a heterotrophic culture where light is not provided, the employment of oxidative stress is essential for promoting the formation of secondary carotenoids including astaxanthin. In the present study, oxidative treatment imposed by chemically generated reactive oxygen species (ROS) including the hydroxyl radicals ((OH)-O-center dot) and singlet oxygen (O-1(2)) for inducing carotenogenesis and astaxanthin production by C. zofingiensis in heterotrophic culture was investigated. (OH)-O-center dot stimulated secondary carotenoids accumulation significantly, whereas O-1(2) did not. The addition of 0.1 mM hydrogen peroxide to the culture leading to the generation of (OH)-O-center dot enhanced the yield of astaxanthin from 9.9 mg l(-1) to 12.58 mg l(-1). These results indicate that the application of a suitable type and dosage of ROS is potentially useful for mass production of astaxanthin by C. zofingiensis in heterotrophic culture. (c) 2005 Elsevier Ltd. All rights reserved.
WOS记录号: WOS:000232393700015
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/2221
Appears in Collections:广东省海洋药物重点实验室_期刊论文

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Ip, PF; Chen, F.Employment of reactive oxygen species to enhance astaxanthin formation in Chlorella zofingiensis in heterotrophic culture,PROCESS BIOCHEMISTRY,2005,40(11):3491-3496
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