SCSIO OpenIR  > 广东省海洋药物重点实验室
Employment of reactive oxygen species to enhance astaxanthin formation in Chlorella zofingiensis in heterotrophic culture
Ip, PF; Chen, F; sfchen@hkusua.hku.hk
2005
Source PublicationPROCESS BIOCHEMISTRY
ISSN1359-5113
Volume40Issue:11Pages:3491-3496
AbstractThe advantages of using heterotrophic cultivation for commercial production of the high-value ketocarotenoid astaxanthin by microalgae have been emphasized in recent years [Chen F. High cell density culture of microalgae in heterotrophic growth. Trends Biotechnol 1996;14:421-5; Chen F, Chen H, Gong X. Mixotrophic and heterotrophic growth of Haematococcus lacustris and rheological behaviour of the cell suspensions. Bioresour Technol 1997;62:19-24; Ip, PF, Chen F. Production of astaxanthin by the green microalga Chlorella zofingiensis in the dark. Process Biochem 2005;40:733-8]. In a heterotrophic culture where light is not provided, the employment of oxidative stress is essential for promoting the formation of secondary carotenoids including astaxanthin. In the present study, oxidative treatment imposed by chemically generated reactive oxygen species (ROS) including the hydroxyl radicals ((OH)-O-center dot) and singlet oxygen (O-1(2)) for inducing carotenogenesis and astaxanthin production by C. zofingiensis in heterotrophic culture was investigated. (OH)-O-center dot stimulated secondary carotenoids accumulation significantly, whereas O-1(2) did not. The addition of 0.1 mM hydrogen peroxide to the culture leading to the generation of (OH)-O-center dot enhanced the yield of astaxanthin from 9.9 mg l(-1) to 12.58 mg l(-1). These results indicate that the application of a suitable type and dosage of ROS is potentially useful for mass production of astaxanthin by C. zofingiensis in heterotrophic culture. (c) 2005 Elsevier Ltd. All rights reserved.
DepartmentUniv Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Peoples R China
KeywordChlorella Zofingiensis Heterotrophic Culture Secondary Carotenoids Astaxanthin Oxidative Stress Reactive Oxygen Species
Subject AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
Indexed Bysci
Funding Project广东省海洋药物重点实验室
WOS IDWOS:000232393700015
Citation statistics
Cited Times:51[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/2221
Collection广东省海洋药物重点实验室
Corresponding Authorsfchen@hkusua.hku.hk
Recommended Citation
GB/T 7714
Ip, PF,Chen, F,sfchen@hkusua.hku.hk. Employment of reactive oxygen species to enhance astaxanthin formation in Chlorella zofingiensis in heterotrophic culture[J]. PROCESS BIOCHEMISTRY,2005,40(11):3491-3496.
APA Ip, PF,Chen, F,&sfchen@hkusua.hku.hk.(2005).Employment of reactive oxygen species to enhance astaxanthin formation in Chlorella zofingiensis in heterotrophic culture.PROCESS BIOCHEMISTRY,40(11),3491-3496.
MLA Ip, PF,et al."Employment of reactive oxygen species to enhance astaxanthin formation in Chlorella zofingiensis in heterotrophic culture".PROCESS BIOCHEMISTRY 40.11(2005):3491-3496.
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