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学科主题: Biotechnology & Applied Microbiology; Marine & Freshwater Biology
题名: Variability of ribosomal DNA ITS-2 and its utility in detecting genetic relatedness of pearl oyster
作者: He, MX ; Huang, LM ; Shi, JH ; Jiang, YP
通讯作者: hmx@scsio.ac.cn
关键词: pearl oyster ; internal transcribed spacer 2 ; variability ; phylogenetic analysis ; species identification
刊名: MARINE BIOTECHNOLOGY
发表日期: 2005
卷: 7, 期:1, 页:40-45
收录类别: sci
部门归属: Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Peoples R China
项目归属: 广东省海洋药物重点实验室
摘要: The objective of this study was to detect interspecific and intraspecific genetic variations of the second internal transcribed spacer of ribosomal DNA (ITS-2), and explore the feasibility of using it as a molecular marker phylogenetic analyses and species identification among pearl oysters. ITS-2 sequences of 6 pearl oysters were amplified via polymerase chain reaction. The amplified DNA fragments were about 500 bp, spanning the partial sequences of 5.8S and 28S rRNA genes. The GC contents of all species used in this study were higher than the AT contents. The variations of sequences involved substitutions as well as insertions/deletions and were mainly concentrated in spacer regions. Sequences of about 30-bp in spacer regions showed no variations among 5 Pincatda species. Intraindividual and intraspecific polymorphisms of ITS-2 sequences were detected in some species; the interspecific variability was significantly larger than the variability within species, and the variability at the genus level was higher than that at the species level. Both neighbor-joining and parsimony analyses of ITS-2 sequences revealed the distinguishable species boundary of 6 pearl oysters, and indicated that P. chemnitzi and P. nigra were the closely related species, as were P. maxima and P. margaritifera. The findings revealed that ITS-2 sequences could be an appropriate tool for phylogenetic study of pearl oysters.
WOS记录号: WOS:000229540200006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/2245
Appears in Collections:广东省海洋药物重点实验室_期刊论文

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He, MX; Huang, LM; Shi, JH; Jiang, YP.Variability of ribosomal DNA ITS-2 and its utility in detecting genetic relatedness of pearl oyster,MARINE BIOTECHNOLOGY,2005,7(1):40-45
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