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学科主题: Biochemical Research Methods; Chemistry, Analytical
题名: Isolation and puritication of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed counter-current chromatography
作者: Li, HB ; Chen, F
通讯作者: sfchen@hkusua.hku.hk
关键词: Scutellaria baicalensis ; counter-current chromatography ; preparative chromatography ; plant materials ; baicalein ; wogonin ; oroxylin A
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2005
卷: 1074, 期:3, 页:107-110
收录类别: sci
部门归属: Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Peoples R China
项目归属: 广东省海洋药物重点实验室
摘要: The medicinal plant Scutellaria baicatensis Georgi has been used widely in traditional Chinese medicine for anti-inflammation, anticancer, antiviral and antibacterial infections, reducing the total cholesterol level and decreasing blood pressures. A high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of three bioactive flavonoids, namely, baicalein, wogonin and oroxylin A, from S. baicalensis Georgi. Preparative HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-n-butanol-water (1: 1: 8: 10, v/v/v/v) was successfully performed by increasing the flow-rate of the mobile phase stepwise from 1.0 to 2.0 ml min(-1) after 4 h. The components purified and collected were analyzed by high-performance liquid chromatography. The method yielded 144.8 mg of baicalein at 95.7% purity, 50.2 mg of wogonin at 98.5% purity, and 12.4 mg of oroxylin A at 93.2% purity from 500 mg of the crude extract in a one-step separation. The recoveries of baicalein, wogonin and oroxylin A were 92.7%, 91.6% and 92.5%, respectively. (c) 2005 Published by Elsevier B.V. All rights reserved.
WOS记录号: WOS:000229192500014
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/2247
Appears in Collections:广东省海洋药物重点实验室_期刊论文

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Li, HB; Chen, F.Isolation and puritication of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed counter-current chromatography,JOURNAL OF CHROMATOGRAPHY A,2005,1074(3):107-110
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文件名: Isolation and purification of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed counter-current chromatography .pdf
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