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学科主题: Biochemical Research Methods; Chemistry, Analytical
题名: Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography
作者: Chen, F ; Li, HB ; Wong, RNS ; Ji, B ; Jiang, Y
通讯作者: sfchen@hkusua.hku.hk
关键词: Microcystis aeruginosa ; counter-current chromatography ; preparative chromatography ; zeaxanthin ; carotenoids
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2005
卷: 1064, 期:2, 页:183-186
收录类别: sci
部门归属: Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Peoples R China; Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China; Hong Kong Baptist Univ, Dept Biol, Kowloon, Hong Kong, Peoples R China
项目归属: 广东省海洋药物重点实验室
摘要: High-speed counter-current chromatography was successfully applied for the first time to the isolation and purification of the bioactive carotenoid zeaxanthin from the cyanobacterium Microcystis aeruginosa. The crude zeaxanthin was obtained by extraction with organic solvents after the microalgal sample had been saponified. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (8:2:7:3, v/v/v/v) was successfully performed yielding zeaxanthin at 96.2% purity from 150 mg of the crude extract in a one-step separation. The recovery of zeaxanthin was 91.4%. This was also the first report that zeaxanthin was successfully separated and purified from microalgae. (C) 2004 Published by Elsevier B.V.
WOS记录号: WOS:000226782100005
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/2255
Appears in Collections:广东省海洋药物重点实验室_期刊论文

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Chen, F; Li, HB; Wong, RNS; Ji, B; Jiang, Y.Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography,JOURNAL OF CHROMATOGRAPHY A,2005,1064(2):183-186
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文件名: Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography .pdf
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