SCSIO OpenIR  > 广东省海洋药物重点实验室
Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography
Chen, F; Li, HB; Wong, RNS; Ji, B; Jiang, Y; sfchen@hkusua.hku.hk
2005
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1064Issue:2Pages:183-186
AbstractHigh-speed counter-current chromatography was successfully applied for the first time to the isolation and purification of the bioactive carotenoid zeaxanthin from the cyanobacterium Microcystis aeruginosa. The crude zeaxanthin was obtained by extraction with organic solvents after the microalgal sample had been saponified. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (8:2:7:3, v/v/v/v) was successfully performed yielding zeaxanthin at 96.2% purity from 150 mg of the crude extract in a one-step separation. The recovery of zeaxanthin was 91.4%. This was also the first report that zeaxanthin was successfully separated and purified from microalgae. (C) 2004 Published by Elsevier B.V.
DepartmentChinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Peoples R China; Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China; Hong Kong Baptist Univ, Dept Biol, Kowloon, Hong Kong, Peoples R China
KeywordMicrocystis Aeruginosa Counter-current Chromatography Preparative Chromatography Zeaxanthin Carotenoids
Subject AreaBiochemical Research Methods ; Chemistry, Analytical
Indexed Bysci
Funding Project广东省海洋药物重点实验室
WOS IDWOS:000226782100005
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/2255
Collection广东省海洋药物重点实验室
Corresponding Authorsfchen@hkusua.hku.hk
Recommended Citation
GB/T 7714
Chen, F,Li, HB,Wong, RNS,et al. Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2005,1064(2):183-186.
APA Chen, F,Li, HB,Wong, RNS,Ji, B,Jiang, Y,&sfchen@hkusua.hku.hk.(2005).Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography.JOURNAL OF CHROMATOGRAPHY A,1064(2),183-186.
MLA Chen, F,et al."Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography".JOURNAL OF CHROMATOGRAPHY A 1064.2(2005):183-186.
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