Development of Fifty-one novel EST-SSR loci in the Pacific oyster, Crassostrea gigas by data mining from the public EST database
Yu, ZN; Wang, YH; Fu, DK; carlzyu@scsio.ac.cn
2010
发表期刊CONSERVATION GENETICS RESOURCES
ISSN1877-7252
卷号2页码:13-18
摘要Fifty-one novel EST-derived microsatellite markers were developed from 23,803 expressed sequence tags in the Pacific oyster, Crassostrea gigas. Polymorphisms of these loci were evaluated in a population of 48 individuals. The allele number ranges from 3 to 11, averaging 5.9 alleles per locus. The observed and expected heterozygosity values range from 0.023 to 0.875 (averaging 0.412) and 0.239 to 0.978 (averaging 0.681), respectively. Twenty-one of the 51 markers accord with Hardy-Weinberg equilibrium (HWE), and the rest show significant departure from HWE after Bonferroni correction, apparently due to heterozygote deficiency, suggesting likely occurrence of null alleles at loci and/or subpopulation of the sample. Cross-amplification was examined in C. hongkongensis and C. arikensis and the results show that 23 and 18 loci, respectively, have a reasonable polymorphism in the two species. These EST-SSR markers will be useful in studies of population genetics, linkage mapping and other aspects for this species.
部门归属[Yu, Ziniu; Wang, Yanhong; Fu, Dingkun] Chinese Acad Sci, S China Sea Inst Oceanol, Lab Marine Bioresource Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China
关键词Crassostrea Gigas Est-ssr Development Polymorphism Cross-species Amplification
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收录类别sci
资助项目LMB
WOS记录号WOS:000290413000004
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被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/3694
专题中科院海洋生物资源可持续利用重点实验室
通讯作者carlzyu@scsio.ac.cn
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Yu, ZN,Wang, YH,Fu, DK,et al. Development of Fifty-one novel EST-SSR loci in the Pacific oyster, Crassostrea gigas by data mining from the public EST database[J]. CONSERVATION GENETICS RESOURCES,2010,2:13-18.
APA Yu, ZN,Wang, YH,Fu, DK,&carlzyu@scsio.ac.cn.(2010).Development of Fifty-one novel EST-SSR loci in the Pacific oyster, Crassostrea gigas by data mining from the public EST database.CONSERVATION GENETICS RESOURCES,2,13-18.
MLA Yu, ZN,et al."Development of Fifty-one novel EST-SSR loci in the Pacific oyster, Crassostrea gigas by data mining from the public EST database".CONSERVATION GENETICS RESOURCES 2(2010):13-18.
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