Isolation and characterization of 24 microsatellite loci in oyster Crassostrea hongkongensis
Li, L; Yu, ZN; carlzyu@scsio.ac.cn
2010
发表期刊CONSERVATION GENETICS RESOURCES
ISSN1877-7252
卷号2页码:93-96
摘要Twenty-four polymorphic microsatellite markers were developed from the Hong Kong oyster, Crassostrea hongkongensis, with a partial genomic library enriched for tandem repeat sequences of (CA)(12), (GA)(12). Polymorphism of these loci was evaluated in a sample of 34 wild unrelated individuals. At these polymorphic loci, the number of alleles per locus vary from 4 to 12, with the average of 7.17; Observed heterozygosity vary from 0.412 to 0.912 with an average of 0.674, while expected values for these loci range from 0.698 to 0.899 with an average of 0.822. After Bonferroni correction (P < 0.002), departure from Hardy-Weinberg equilibrium are observed in four loci (Ch210, Ch211, Ch214 and Ch217) due to homozygote excess and not detected in others. Additionally, four loci (Ch209 and Ch211, Ch207 and Ch224) showed significant linkage disequilibrium (P < 0.01). This set of microsatellite loci would be useful for stock and genetic studies in C. hongkongensis.
部门归属[Li, Lu; Yu, Ziniu] Chinese Acad Sci, S China Sea Inst Oceanol, Labs Appl Marine Biol & Marine Bioresource Sustai, Guangzhou 510301, Guangdong, Peoples R China
关键词Crassostrea Hongkongensis Microsatellites Isolation Polymorphism
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收录类别sci
资助项目LMB
WOS记录号WOS:000290413000023
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被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/3696
专题中科院海洋生物资源可持续利用重点实验室
通讯作者carlzyu@scsio.ac.cn
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Li, L,Yu, ZN,carlzyu@scsio.ac.cn. Isolation and characterization of 24 microsatellite loci in oyster Crassostrea hongkongensis[J]. CONSERVATION GENETICS RESOURCES,2010,2:93-96.
APA Li, L,Yu, ZN,&carlzyu@scsio.ac.cn.(2010).Isolation and characterization of 24 microsatellite loci in oyster Crassostrea hongkongensis.CONSERVATION GENETICS RESOURCES,2,93-96.
MLA Li, L,et al."Isolation and characterization of 24 microsatellite loci in oyster Crassostrea hongkongensis".CONSERVATION GENETICS RESOURCES 2(2010):93-96.
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