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学科主题: Fisheries
题名: Microbiota of yellow grouper (Epinephelus awoora Temminck & Schlegel, 1842) fed two different diets
作者: Feng, JB ; Hu, CQ ; Luo, P ; Zhang, LP ; Chen, C
通讯作者: cqhu@scsio.ac.cn
关键词: yellow grouper ; microbiota ; DGGE ; skin ; gill ; intestinal-non-adherent bacteria ; intestinal-adherent bacteria
刊名: AQUACULTURE RESEARCH
发表日期: 2010
卷: 41, 期:12, 页:1778-1790
收录类别: sci
部门归属: [Feng, Jing-Bin; Hu, Chao-Qun; Luo, Peng; Zhang, Lv-Ping; Chen, Chang] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Feng, Jing-Bin; Hu, Chao-Qun; Luo, Peng; Zhang, Lv-Ping; Chen, Chang] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China; [Feng, Jing-Bin; Hu, Chao-Qun; Luo, Peng; Zhang, Lv-Ping; Chen, Chang] Chinese Acad Sci, S China Sea Inst Oceanol, Chinese Acad Sci LAMB, Guangzhou 510301, Guangdong, Peoples R China; [Feng, Jing-Bin] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
项目归属: LMB
摘要: The rapidly growing yellow grouper industry has experienced relatively severe bacterial disease problems in China. The proliferation of pathogens in fish can be suppressed by commensal microbiota. In this background, we used nested polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and sequence analysis to investigate microbiota in the skin, gills and intestines, including adherent bacteria and non-adherent bacteria in yellow grouper fed with natural diet and complete feed. A total of 21 bacterial species were identified using phylogenetic analysis. The gamma-Proteobacteria group (81.0%, 17 species) dominated the bacterial communities in yellow grouper completely. Others belonged to Firmicutes (9.5%, two species), Actinobacteria (4.75%, one species) and Verrucomicrobia (4.75%, one species). The higher similarities (above 91%) of the DGGE band patterns in skin, gill and intestinal-non-adherent bacteria between two groups of fish indicated that existed more stable microbial communities existed in these specifically ecological niches in yellow grouper. However, considerable differences existed between two intestinal-adherent bacteria (IAB) samples; that is, compared with natural diet fed yellow grouper, higher bacterial apparent species richness and possibly less abundance existed in IAB in fish fed with complete diets, probably indicating that the community structures in IAB were affected easily and significantly by diet.
原文出处: 查看原文
WOS记录号: WOS:000283946200006
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/3732
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Feng, JB; Hu, CQ; Luo, P; Zhang, LP; Chen, C.Microbiota of yellow grouper (Epinephelus awoora Temminck & Schlegel, 1842) fed two different diets,AQUACULTURE RESEARCH,2010,41(12):1778-1790
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