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Microbiota of yellow grouper (Epinephelus awoora Temminck & Schlegel, 1842) fed two different diets
Feng, JB; Hu, CQ; Luo, P; Zhang, LP; Chen, C; cqhu@scsio.ac.cn
2010
Source PublicationAQUACULTURE RESEARCH
ISSN1355-557X
Volume41Issue:12Pages:1778-1790
AbstractThe rapidly growing yellow grouper industry has experienced relatively severe bacterial disease problems in China. The proliferation of pathogens in fish can be suppressed by commensal microbiota. In this background, we used nested polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and sequence analysis to investigate microbiota in the skin, gills and intestines, including adherent bacteria and non-adherent bacteria in yellow grouper fed with natural diet and complete feed. A total of 21 bacterial species were identified using phylogenetic analysis. The gamma-Proteobacteria group (81.0%, 17 species) dominated the bacterial communities in yellow grouper completely. Others belonged to Firmicutes (9.5%, two species), Actinobacteria (4.75%, one species) and Verrucomicrobia (4.75%, one species). The higher similarities (above 91%) of the DGGE band patterns in skin, gill and intestinal-non-adherent bacteria between two groups of fish indicated that existed more stable microbial communities existed in these specifically ecological niches in yellow grouper. However, considerable differences existed between two intestinal-adherent bacteria (IAB) samples; that is, compared with natural diet fed yellow grouper, higher bacterial apparent species richness and possibly less abundance existed in IAB in fish fed with complete diets, probably indicating that the community structures in IAB were affected easily and significantly by diet.
Department[Feng, Jing-Bin; Hu, Chao-Qun; Luo, Peng; Zhang, Lv-Ping; Chen, Chang] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Feng, Jing-Bin; Hu, Chao-Qun; Luo, Peng; Zhang, Lv-Ping; Chen, Chang] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China; [Feng, Jing-Bin; Hu, Chao-Qun; Luo, Peng; Zhang, Lv-Ping; Chen, Chang] Chinese Acad Sci, S China Sea Inst Oceanol, Chinese Acad Sci LAMB, Guangzhou 510301, Guangdong, Peoples R China; [Feng, Jing-Bin] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
KeywordYellow Grouper Microbiota Dgge Skin Gill Intestinal-non-adherent Bacteria Intestinal-adherent Bacteria
Subject AreaFisheries
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Indexed Bysci
Funding ProjectLMB
WOS IDWOS:000283946200006
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/3732
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorcqhu@scsio.ac.cn
Recommended Citation
GB/T 7714
Feng, JB,Hu, CQ,Luo, P,et al. Microbiota of yellow grouper (Epinephelus awoora Temminck & Schlegel, 1842) fed two different diets[J]. AQUACULTURE RESEARCH,2010,41(12):1778-1790.
APA Feng, JB,Hu, CQ,Luo, P,Zhang, LP,Chen, C,&cqhu@scsio.ac.cn.(2010).Microbiota of yellow grouper (Epinephelus awoora Temminck & Schlegel, 1842) fed two different diets.AQUACULTURE RESEARCH,41(12),1778-1790.
MLA Feng, JB,et al."Microbiota of yellow grouper (Epinephelus awoora Temminck & Schlegel, 1842) fed two different diets".AQUACULTURE RESEARCH 41.12(2010):1778-1790.
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