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学科主题: Fisheries; Marine & Freshwater Biology
题名: Establishment and characterization of a new marine fish cell line derived from red-spotted grouper Epinephelus akaara
作者: Ou-Yang, ZL ; Huang, XH ; Huang, EY ; Huang, YH ; Gong, J ; Sun, JJ ; Qin, QW
通讯作者: qinqw@scsio.ac.cn
关键词: cell line ; Epinephelus akaara ; grouper liver ; virus
刊名: JOURNAL OF FISH BIOLOGY
发表日期: 2010
卷: 77, 期:5, 页:1083-1095
收录类别: sci
部门归属: [Huang, X. H.; Huang, Y. H.; Qin, Q. W.] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Ou-Yang, Z. L.; Huang, E. Y.; Gong, J.; Sun, J. J.] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China
项目归属: LMB
摘要: A new marine fish cell line, EAGL, derived from the liver of red-spotted grouper Epinephelus akaara was established and characterized. The cells multiplied well in minimum essential medium (MEM) supplemented with 10% foetal bovine serum (FBS) at temperatures between 25 and 30 degrees C. The growth rate of this cell line increased as the proportion of FBS increased from 5 to 20% at 25 degrees C, with maximum growth at the concentration of 15 or 20% FBS. Morphologically, the cells were epithelial-like and the presence of pancytokeratin confirmed their epithelial origin. Chromosome analysis revealed that the modal chromosome number was 48. The susceptibility of the cell line to four fish viruses was tested. Significant cytopathic effect (CPE) was only observed in Singapore grouper iridovirus (SGIV)-infected cells, and the virus replication was further confirmed by immunofluorescence, electron microscopy and real-time reverse-transcription (RT)-PCR assay. When the cells were transfected with pEGFP-N3 plasmid, bright fluorescent signals were observed, suggesting that this cell line can be used for transgenic and genetic manipulation studies.
原文出处: 查看原文
WOS记录号: WOS:000282570600003
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/3748
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Ou-Yang, ZL; Huang, XH; Huang, EY; Huang, YH; Gong, J; Sun, JJ; Qin, QW.Establishment and characterization of a new marine fish cell line derived from red-spotted grouper Epinephelus akaara,JOURNAL OF FISH BIOLOGY,2010,77(5):1083-1095
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