中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室  > 期刊论文
学科主题: Biotechnology & Applied Microbiology; Microbiology
题名: Immunoproteomic analysis and identification of novel immunogenic proteins from Vibrio harveyi
作者: Pang, HY ; Li, Y ; Wu, ZH ; Jian, JC ; Lu, YS ; Cai, SH
通讯作者: wuzh@gdou.edu.cn
关键词: immunoproteome ; Vibrio harveyi ; whole-cell protein
刊名: JOURNAL OF APPLIED MICROBIOLOGY
发表日期: 2010
卷: 109, 期:5, 页:1800-1809
收录类别: sci
部门归属: [Pang, H. -Y.; Li, Y.; Wu, Z. -H.; Jian, J. -C.; Lu, Y. -S.; Cai, S. -H.] Guangdong Ocean Univ, Coll Fisheries, Zhanjiang City 524025, Guangdong, Peoples R China; [Pang, H. -Y.] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China; [Pang, H. -Y.] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China; [Pang, H. -Y.; Li, Y.; Wu, Z. -H.; Jian, J. -C.; Lu, Y. -S.; Cai, S. -H.] Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China; [Pang, H. -Y.; Li, Y.; Wu, Z. -H.; Jian, J. -C.; Lu, Y. -S.; Cai, S. -H.] Guangdong Higher Educ Inst, Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China
项目归属: LMB
摘要: Aims: The main aim of this study was to screen novel immunogenic proteins of Vibrio harveyi, which could be vaccine candidates. Methods and Results: Whole-cell proteins of V. harveyi, strain Li01 and Huang01, were first separated by isoelectric focusing, followed by 2D-PAGE, respectively. Immunogenic proteins were identified by Western blotting, using Epinephelus coioides antisera against V. harveyi strain Li01. Western blot analyses revealed 16 shared immunogenic protein spots in both strains. All of the immunogenic proteins were successfully identified and corresponded to 15 proteins. None of these proteins have been previously reported as immunogenic for V. harveyi. Of the 15 proteins, 11 are specific immunoreactive proteins and four are nonspecific immunoreactive proteins. Furthermore, outer membrane protein N (spot 2) and oligopeptide ATP-binding cassette (ABC) transporter (spot 3) were used as immunogens to immunize E. coioides for investigation of their protective abilities and activities. The E. coioides immunized with OmpN has abilities to fight against infections caused by V. harveyi Li01 and Huang01. However, vaccination with oligopeptide ABC transporter induces low protective immune response in fish. Conclusions: Eleven novel specific antigens were found, and OmpN could potentially be used as vaccine candidate for the development of novel vaccine against V. harveyi. Significance and Impact of the Study: These data show that immunoproteomics methods can be successfully applied in identifying immunogenic proteins of V. harveyi, which helps to search for the protective antigens in future.
原文出处: 查看原文
WOS记录号: WOS:000282813800031
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/3750
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Immunoproteomic analysis and identification of novel immunogenic proteins from Vibrio harveyi.pdf(317KB)----开放获取--View Download

Recommended Citation:
Pang, HY; Li, Y; Wu, ZH; Jian, JC; Lu, YS; Cai, SH.Immunoproteomic analysis and identification of novel immunogenic proteins from Vibrio harveyi,JOURNAL OF APPLIED MICROBIOLOGY,2010,109(5):1800-1809
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Pang, HY]'s Articles
[Li, Y]'s Articles
[Wu, ZH]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Pang, HY]‘s Articles
[Li, Y]‘s Articles
[Wu, ZH]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Immunoproteomic analysis and identification of novel immunogenic proteins from Vibrio harveyi.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace