Immunoproteomic analysis and identification of novel immunogenic proteins from Vibrio harveyi
Pang, HY; Li, Y; Wu, ZH; Jian, JC; Lu, YS; Cai, SH; wuzh@gdou.edu.cn
2010
发表期刊JOURNAL OF APPLIED MICROBIOLOGY
ISSN1364-5072
卷号109期号:5页码:1800-1809
摘要Aims: The main aim of this study was to screen novel immunogenic proteins of Vibrio harveyi, which could be vaccine candidates. Methods and Results: Whole-cell proteins of V. harveyi, strain Li01 and Huang01, were first separated by isoelectric focusing, followed by 2D-PAGE, respectively. Immunogenic proteins were identified by Western blotting, using Epinephelus coioides antisera against V. harveyi strain Li01. Western blot analyses revealed 16 shared immunogenic protein spots in both strains. All of the immunogenic proteins were successfully identified and corresponded to 15 proteins. None of these proteins have been previously reported as immunogenic for V. harveyi. Of the 15 proteins, 11 are specific immunoreactive proteins and four are nonspecific immunoreactive proteins. Furthermore, outer membrane protein N (spot 2) and oligopeptide ATP-binding cassette (ABC) transporter (spot 3) were used as immunogens to immunize E. coioides for investigation of their protective abilities and activities. The E. coioides immunized with OmpN has abilities to fight against infections caused by V. harveyi Li01 and Huang01. However, vaccination with oligopeptide ABC transporter induces low protective immune response in fish. Conclusions: Eleven novel specific antigens were found, and OmpN could potentially be used as vaccine candidate for the development of novel vaccine against V. harveyi. Significance and Impact of the Study: These data show that immunoproteomics methods can be successfully applied in identifying immunogenic proteins of V. harveyi, which helps to search for the protective antigens in future.
部门归属[Pang, H. -Y.; Li, Y.; Wu, Z. -H.; Jian, J. -C.; Lu, Y. -S.; Cai, S. -H.] Guangdong Ocean Univ, Coll Fisheries, Zhanjiang City 524025, Guangdong, Peoples R China; [Pang, H. -Y.] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China; [Pang, H. -Y.] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China; [Pang, H. -Y.; Li, Y.; Wu, Z. -H.; Jian, J. -C.; Lu, Y. -S.; Cai, S. -H.] Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China; [Pang, H. -Y.; Li, Y.; Wu, Z. -H.; Jian, J. -C.; Lu, Y. -S.; Cai, S. -H.] Guangdong Higher Educ Inst, Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China
关键词Immunoproteome Vibrio Harveyi Whole-cell Protein
学科领域Biotechnology & Applied Microbiology ; Microbiology
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收录类别sci
资助项目LMB
WOS记录号WOS:000282813800031
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/3750
专题中科院海洋生物资源可持续利用重点实验室
通讯作者wuzh@gdou.edu.cn
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Pang, HY,Li, Y,Wu, ZH,et al. Immunoproteomic analysis and identification of novel immunogenic proteins from Vibrio harveyi[J]. JOURNAL OF APPLIED MICROBIOLOGY,2010,109(5):1800-1809.
APA Pang, HY.,Li, Y.,Wu, ZH.,Jian, JC.,Lu, YS.,...&wuzh@gdou.edu.cn.(2010).Immunoproteomic analysis and identification of novel immunogenic proteins from Vibrio harveyi.JOURNAL OF APPLIED MICROBIOLOGY,109(5),1800-1809.
MLA Pang, HY,et al."Immunoproteomic analysis and identification of novel immunogenic proteins from Vibrio harveyi".JOURNAL OF APPLIED MICROBIOLOGY 109.5(2010):1800-1809.
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