Genetic characterization of ticks from southwestern Romania by sequences of mitochondrial cox1 and nad5 genes
Chitimia, L; Lin, RQ; Cosoroaba, I; Wu, XY; Song, HQ; Yuan, ZG; Zhu, XQ; rqlin@scau.edu.cn; xingquanzh@scau.edu.cn
2010
发表期刊EXPERIMENTAL AND APPLIED ACAROLOGY
ISSN0168-8162
卷号52期号:3页码:305-311
摘要In the present study, samples representing three hard tick species and one soft tick species, namely Dermacentor marginatus, Haemaphysalis punctata, Ixodes ricinus and Argas persicus from southwestern Romania, and one hard tick, Haemaphysalis longicornis, from China were characterized genetically by a portion of mitochondrial cytochrome c oxidase subunit 1 gene (pcox1) and a portion of nicotinamide adenine dinucleotide dehydrogenase subunit 5 gene (pnad5). The pcox1 and pnad5 were amplified separately from individual ticks by PCR, sequenced and analyzed. The length of pcox1 and pnad5 sequences of all samples was 732 and 519 bp, respectively. The intra-specific sequence variation in De. marginatus was 0.1-1.0% for pcox1 and 0.2-1.2% for pnad5, whereas in Ha. punctata it was 0.4-1.9% for pcox1 and 0.4-1.0% for pnad5. For the tick species examined in the present study, sequence comparison revealed that the inter-specific sequence differences were higher: 15.9-27.6% for pcox1 and 20.3-42.4% for pnad5. This suggests that the cox1 and nad5 sequences could provide useful genetic markers for the specific identification and genetic characterization of ticks in Romania and elsewhere.
部门归属[Chitimia, Lidia; Lin, Rui-Qing; Song, Hui-Qun; Yuan, Zi-Guo; Zhu, Xing-Quan] S China Agr Univ, Coll Vet Med, Dept Parasitol, Guangzhou 510642, Guangdong, Peoples R China; [Cosoroaba, Iustin] Fac Vet Med, Dept Parasitol, Timisoara 300645, Romania; [Wu, Xiang-Yun] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Chitimia, Lidia] Inst Diag & Anim Hlth, Bucharest 050557, Romania; [Zhu, Xing-Quan] CAAS, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou Vet Res Inst, Lanzhou 730046, Gansu, Peoples R China
关键词Ticks Cytochrome c Oxidase Subunit 1 Gene (Cox1) Nicotinamide Adenine Dinucleotide Dehydrogenase Subunit 5 Gene (Nad5) Phylogenetic Analysis Romania Mitochondrial Dna
学科领域Entomology
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收录类别sci
资助项目LMB
WOS记录号WOS:000282701300006
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/3752
专题中科院海洋生物资源可持续利用重点实验室
通讯作者rqlin@scau.edu.cn; xingquanzh@scau.edu.cn
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Chitimia, L,Lin, RQ,Cosoroaba, I,et al. Genetic characterization of ticks from southwestern Romania by sequences of mitochondrial cox1 and nad5 genes[J]. EXPERIMENTAL AND APPLIED ACAROLOGY,2010,52(3):305-311.
APA Chitimia, L.,Lin, RQ.,Cosoroaba, I.,Wu, XY.,Song, HQ.,...&xingquanzh@scau.edu.cn.(2010).Genetic characterization of ticks from southwestern Romania by sequences of mitochondrial cox1 and nad5 genes.EXPERIMENTAL AND APPLIED ACAROLOGY,52(3),305-311.
MLA Chitimia, L,et al."Genetic characterization of ticks from southwestern Romania by sequences of mitochondrial cox1 and nad5 genes".EXPERIMENTAL AND APPLIED ACAROLOGY 52.3(2010):305-311.
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