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学科主题: Entomology
题名: Genetic characterization of ticks from southwestern Romania by sequences of mitochondrial cox1 and nad5 genes
作者: Chitimia, L ; Lin, RQ ; Cosoroaba, I ; Wu, XY ; Song, HQ ; Yuan, ZG ; Zhu, XQ
通讯作者: rqlin@scau.edu.cn ; xingquanzh@scau.edu.cn
关键词: Ticks ; Cytochrome c oxidase subunit 1 gene (cox1) ; Nicotinamide adenine dinucleotide dehydrogenase subunit 5 gene (nad5) ; Phylogenetic analysis ; Romania ; Mitochondrial DNA
刊名: EXPERIMENTAL AND APPLIED ACAROLOGY
发表日期: 2010
卷: 52, 期:3, 页:305-311
收录类别: sci
部门归属: [Chitimia, Lidia; Lin, Rui-Qing; Song, Hui-Qun; Yuan, Zi-Guo; Zhu, Xing-Quan] S China Agr Univ, Coll Vet Med, Dept Parasitol, Guangzhou 510642, Guangdong, Peoples R China; [Cosoroaba, Iustin] Fac Vet Med, Dept Parasitol, Timisoara 300645, Romania; [Wu, Xiang-Yun] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Chitimia, Lidia] Inst Diag & Anim Hlth, Bucharest 050557, Romania; [Zhu, Xing-Quan] CAAS, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou Vet Res Inst, Lanzhou 730046, Gansu, Peoples R China
项目归属: LMB
摘要: In the present study, samples representing three hard tick species and one soft tick species, namely Dermacentor marginatus, Haemaphysalis punctata, Ixodes ricinus and Argas persicus from southwestern Romania, and one hard tick, Haemaphysalis longicornis, from China were characterized genetically by a portion of mitochondrial cytochrome c oxidase subunit 1 gene (pcox1) and a portion of nicotinamide adenine dinucleotide dehydrogenase subunit 5 gene (pnad5). The pcox1 and pnad5 were amplified separately from individual ticks by PCR, sequenced and analyzed. The length of pcox1 and pnad5 sequences of all samples was 732 and 519 bp, respectively. The intra-specific sequence variation in De. marginatus was 0.1-1.0% for pcox1 and 0.2-1.2% for pnad5, whereas in Ha. punctata it was 0.4-1.9% for pcox1 and 0.4-1.0% for pnad5. For the tick species examined in the present study, sequence comparison revealed that the inter-specific sequence differences were higher: 15.9-27.6% for pcox1 and 20.3-42.4% for pnad5. This suggests that the cox1 and nad5 sequences could provide useful genetic markers for the specific identification and genetic characterization of ticks in Romania and elsewhere.
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WOS记录号: WOS:000282701300006
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/3752
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Chitimia, L; Lin, RQ; Cosoroaba, I; Wu, XY; Song, HQ; Yuan, ZG; Zhu, XQ.Genetic characterization of ticks from southwestern Romania by sequences of mitochondrial cox1 and nad5 genes,EXPERIMENTAL AND APPLIED ACAROLOGY,2010,52(3):305-311
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