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学科主题: Microbiology
题名: The type III secretion system of Vibrio alginolyticus induces rapid apoptosis, cell rounding and osmotic lysis of fish cells
作者: Zhao, Z ; Chen, C ; Hu, CQ ; Ren, CH ; Zhao, JJ ; Zhang, LP ; Jiang, XA ; Luo, P ; Wang, QB
通讯作者: cqhu@scsio.ac.cn
刊名: MICROBIOLOGY-SGM
发表日期: 2010
卷: 156, 页:2864-2872
收录类别: sci
部门归属: [Hu, Chao-Qun] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov LAMB, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
摘要: Vibrio alginolyticus is a Gram-negative bacterium and has been recognized as an opportunistic pathogen in humans as well as marine animals. However, the virulence mechanisms for this species of Vibrio have not been elucidated. This study characterized multiple mechanisms that induce cell death in fish cells upon infection with a V. alginolyticus strain, ZJO. The bacterium required its type III secretion system (T3SS) to cause rapid death of infected fish cells. Dying cells exhibited some features of apoptotic cells, such as membrane blebbing, nuclear condensation and DNA fragmentation. Further studies showed that caspase-3 was activated by the T3SS of the ZJO strain, confirming that infection with V. alginolyticus rapidly induces T3SS-dependent apoptosis in fish cells. Infection with the ZJO strain also led to membrane pore formation and release of cellular contents from infected fish cells, as evidenced by lactate dehydrogenase release and the uptake of a membrane-impermeable dye. Importantly, inhibition of apoptosis did not prevent ZJO-infected cells from releasing cellular contents and did not block cell rounding. Taken together, these data demonstrate that infection with V. alginolyticus may promote at least three different T3SS-dependent events, which lead to the death of fish cells. This study provides an important insight into the mechanism used by Vibrio species to cause host-cell death.
原文出处: 查看原文
WOS记录号: WOS:000282565700026
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/3754
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Zhao, Z; Chen, C; Hu, CQ; Ren, CH; Zhao, JJ; Zhang, LP; Jiang, XA; Luo, P; Wang, QB.The type III secretion system of Vibrio alginolyticus induces rapid apoptosis, cell rounding and osmotic lysis of fish cells,MICROBIOLOGY-SGM,2010,156():2864-2872
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