In silico cloning, expression of Rieske-like apoprotein gene and protein subcellular localization in the Pacific oyster, Crassostrea gigas
He, XC; Zhang, Y; Yu, ZN; carlzyu@scsio.ac.cn
2010
发表期刊MOLECULAR BIOLOGY REPORTS
ISSN0301-4851
卷号37期号:7页码:3259-3264
摘要Rieske protein gene in the Pacific oyster Crassostrea gigas was obtained by in silico cloning for the first time, and its expression profiles and subcellular localization were determined, respectively. The full-length cDNA of Cgisp is 985 bp in length and contains a 5'- and 3'-untranslated regions of 35 and 161 bp, respectively, with an open reading frame of 786 bp encoding a protein of 262 amino acids. The predicted molecular weight of 30 kDa of Cgisp protein was verified by prokaryotic expression. Conserved Rieske [2Fe-2S] cluster binding sites and highly matched-pair tertiary structure with 3CWB_E (Gallus gallus) were revealed by homologous analysis and molecular modeling. Eleven putative SNP sites and two conserved hexapeptide sequences, box I (THLGC) and II (PCHGS), were detected by multiple alignments. Real-time PCR analysis showed that Cgisp is expressed in a wide range of tissues, with adductor muscle exhibiting the top expression level, suggesting its biological function of energy transduction. The GFP tagging Cgisp indicated a mitochondrial localization, further confirming its physiological function.
部门归属[He, Xiaocui; Zhang, Yang; Yu, Ziniu] Chinese Acad Sci, Lab Marine Bioresource Sustainable Utilizat, Lab Appl Marine Biol, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
关键词Crassostrea Gigas In Silico clonIng Rieske Apoprotein Gene Expression Patterns Characterization Subcellular Localization
学科领域Biochemistry & Molecular Biology
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收录类别sci
资助项目LMB
WOS记录号WOS:000281985400028
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/3770
专题中科院海洋生物资源可持续利用重点实验室
通讯作者carlzyu@scsio.ac.cn
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He, XC,Zhang, Y,Yu, ZN,et al. In silico cloning, expression of Rieske-like apoprotein gene and protein subcellular localization in the Pacific oyster, Crassostrea gigas[J]. MOLECULAR BIOLOGY REPORTS,2010,37(7):3259-3264.
APA He, XC,Zhang, Y,Yu, ZN,&carlzyu@scsio.ac.cn.(2010).In silico cloning, expression of Rieske-like apoprotein gene and protein subcellular localization in the Pacific oyster, Crassostrea gigas.MOLECULAR BIOLOGY REPORTS,37(7),3259-3264.
MLA He, XC,et al."In silico cloning, expression of Rieske-like apoprotein gene and protein subcellular localization in the Pacific oyster, Crassostrea gigas".MOLECULAR BIOLOGY REPORTS 37.7(2010):3259-3264.
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