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Staphylococcus aureus promoter-lux reporters for drug discovery
Mesak, LR; Qi, SH; Villanueva, I; Miao, V; Davies, J
AbstractWe describe a collection of antibiotic-activated Staphylococcus aureus promoter-lux reporter strains that can be used to discriminate among antibiotic classes on the basis of their light production response profile. We screened over 400 culture supernatants from previously uncharacterized actinomycetes from soil for the production of aminocoumarin-type compounds and DNA-damaging agents. Novobiocin production was determined in three isolates of Streptomyces, and streptonigrin, a DNA-damaging agent, together with several other bioactive compounds (oxopropaline D and G), was identified from a novel Kitasatospora isolate. This array provides an effective and specific whole-cell approach to search for classes of antimicrobial compounds in unfractionated culture broths. The Journal of Antibiotics (2010) 63, 492-498, doi 10 1038/ja 2010 74, published online 7 July 2010
Department[Mesak, Lili R.; Villanueva, Ivan; Miao, Vivian; Davies, Julian] Univ British Columbia, Inst Life Sci, Dept Microbiol & Immunol, Vancouver, BC V6T 1Z3, Canada; [Qi, Shuhua] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangdong Key Lab Marine Mat Med, Guangzhou, Guangdong, Peoples R China
KeywordActinomycete Antibiotic Profiling/fingerprint Luciferase Promoter-lux Reporter Staphylococcus Aureus
Subject AreaBiotechnology & Applied Microbiology ; Immunology ; Microbiology ; Pharmacology & Pharmacy
Indexed Bysci
Funding ProjectLMB
WOS IDWOS:000281499800013
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
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GB/T 7714
Mesak, LR,Qi, SH,Villanueva, I,et al. Staphylococcus aureus promoter-lux reporters for drug discovery[J]. JOURNAL OF ANTIBIOTICS,2010,63(8):492-498.
APA Mesak, LR,Qi, SH,Villanueva, I,Miao, V,&Davies, J.(2010).Staphylococcus aureus promoter-lux reporters for drug discovery.JOURNAL OF ANTIBIOTICS,63(8),492-498.
MLA Mesak, LR,et al."Staphylococcus aureus promoter-lux reporters for drug discovery".JOURNAL OF ANTIBIOTICS 63.8(2010):492-498.
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