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Differential profiles of gene expression in grouper Epinephelus coioides, infected with Singapore grouper iridovirus, revealed by suppression subtractive hybridization and DNA microarray
Xu, D; Wei, J; Cui, H; Gong, J; Yan, Y; Lai, R; Qin, Q; qinqw@scsio.ac.cn
2010
Source PublicationJOURNAL OF FISH BIOLOGY
ISSN0022-1112
Volume77Issue:2Pages:341-360
AbstractSuppression subtractive hybridization (SSH) was used to generate a subtracted cDNA library enriched with gene transcripts differentially expressed in the spleen of orange-spotted grouper Epinephelus coioides after 5 days of infection with Singapore grouper iridovirus (SGIV). In the forward and reverse-subtracted libraries, 260 and 153 non-redundant expressed sequence tags (EST), respectively, were identified. These annotated genes responding to SGIV infection were grouped into eight gene categories related to immunity, cell structure, transcription-translation, cell signalling, metabolism, mitochondrial proteins, ribosomal proteins and unknown or hypothetical proteins. A DNA microarray containing all the differentially expressed genes was constructed, and the gene expression patterns in different tissues were investigated in virus-infected E. coioides. Of these genes, four associated with the infection processes were identified and further investigated by quantitative real-time PCR. These results provide new insights into the molecular basis of host-pathogen interactions in E. coioides, and will help the development of control strategies against SGIV infection.
Department[Qin, Q.] S China Sea Inst Oceanol, Key Lab Marine Bioresourses Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Xu, D.; Wei, J.; Cui, H.; Gong, J.; Yan, Y.; Lai, R.] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China
KeywordDifferential Gene Expression Epinephelus Coioides Microarray Singapore Grouper Iridovirus Suppression Subtractive Hybridization
Subject AreaFisheries ; Marine & Freshwater Biology
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Indexed Bysci
Funding ProjectLMB
WOS IDWOS:000280151300003
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Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/3794
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorqinqw@scsio.ac.cn
Recommended Citation
GB/T 7714
Xu, D,Wei, J,Cui, H,et al. Differential profiles of gene expression in grouper Epinephelus coioides, infected with Singapore grouper iridovirus, revealed by suppression subtractive hybridization and DNA microarray[J]. JOURNAL OF FISH BIOLOGY,2010,77(2):341-360.
APA Xu, D.,Wei, J.,Cui, H.,Gong, J.,Yan, Y.,...&qinqw@scsio.ac.cn.(2010).Differential profiles of gene expression in grouper Epinephelus coioides, infected with Singapore grouper iridovirus, revealed by suppression subtractive hybridization and DNA microarray.JOURNAL OF FISH BIOLOGY,77(2),341-360.
MLA Xu, D,et al."Differential profiles of gene expression in grouper Epinephelus coioides, infected with Singapore grouper iridovirus, revealed by suppression subtractive hybridization and DNA microarray".JOURNAL OF FISH BIOLOGY 77.2(2010):341-360.
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