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学科主题: Fisheries; Marine & Freshwater Biology
题名: Differential profiles of gene expression in grouper Epinephelus coioides, infected with Singapore grouper iridovirus, revealed by suppression subtractive hybridization and DNA microarray
作者: Xu, D ; Wei, J ; Cui, H ; Gong, J ; Yan, Y ; Lai, R ; Qin, Q
通讯作者: qinqw@scsio.ac.cn
关键词: differential gene expression ; Epinephelus coioides ; microarray ; Singapore grouper iridovirus ; suppression subtractive hybridization
刊名: JOURNAL OF FISH BIOLOGY
发表日期: 2010
卷: 77, 期:2, 页:341-360
收录类别: sci
部门归属: [Qin, Q.] S China Sea Inst Oceanol, Key Lab Marine Bioresourses Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Xu, D.; Wei, J.; Cui, H.; Gong, J.; Yan, Y.; Lai, R.] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China
项目归属: LMB
摘要: Suppression subtractive hybridization (SSH) was used to generate a subtracted cDNA library enriched with gene transcripts differentially expressed in the spleen of orange-spotted grouper Epinephelus coioides after 5 days of infection with Singapore grouper iridovirus (SGIV). In the forward and reverse-subtracted libraries, 260 and 153 non-redundant expressed sequence tags (EST), respectively, were identified. These annotated genes responding to SGIV infection were grouped into eight gene categories related to immunity, cell structure, transcription-translation, cell signalling, metabolism, mitochondrial proteins, ribosomal proteins and unknown or hypothetical proteins. A DNA microarray containing all the differentially expressed genes was constructed, and the gene expression patterns in different tissues were investigated in virus-infected E. coioides. Of these genes, four associated with the infection processes were identified and further investigated by quantitative real-time PCR. These results provide new insights into the molecular basis of host-pathogen interactions in E. coioides, and will help the development of control strategies against SGIV infection.
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WOS记录号: WOS:000280151300003
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/3794
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Xu, D; Wei, J; Cui, H; Gong, J; Yan, Y; Lai, R; Qin, Q.Differential profiles of gene expression in grouper Epinephelus coioides, infected with Singapore grouper iridovirus, revealed by suppression subtractive hybridization and DNA microarray,JOURNAL OF FISH BIOLOGY,2010,77(2):341-360
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