Deletion of valR, a homolog of Vibrio harveyis luxR generates an intermediate colony phenotype between opaque/rugose and translucent/smooth in Vibrio alginolyticus
Chen, C; Zhao, JJ; Ren, CH; Hu, CQ; chen.chang@scsio.ac.cn; cqhu@scsio.ac.cn
2010
发表期刊BIOFOULING
ISSN0892-7014
卷号26期号:5页码:595-601
摘要A previous study has shown that Vibrio alginolyticus ZJ-51 undergoes colony phase variation between opaque/ rugose (Op) and translucent/smooth (Tr). The AI-2 quorum-sensing master regulator ValR, a homolog to V. harveyi LuxR, was suggested to be involved in the transition. To investigate the role of ValR in the variation and in biofilm formation, an in-frame deletion of valR in both Op and Tr backgrounds was carried out. The mutants in both backgrounds showed an intermediate colony morphotype, where the colonies were less opaque/rugose but not fully translucent/smooth either. They also showed an intermediate level of motility. However, biofilm formation was severely decreased in both mutants and polar flagella were depleted also. Quantitative PCR showed that most of the genes related to flagellar and polysaccharide biosynthesis were upregulated in the mutant of Op background (DvalR/Op) but downregulated in the mutant of Tr background (DvalR/Tr) compared with their parental wild-type strains. This suggests that ValR may control biofilm formation by regulating flagellar biosynthesis and affect the expression of the genes involved in colony phase variation in V. alginolyticus.
部门归属[Chen Chang] CAS, LMB, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; CAS, S China Sea Inst Oceanol, LAMB, Guangzhou 510301, Guangdong, Peoples R China
关键词Vibrio Alginolyticus Biofilm Formation Quorum Sensing Colony Phase Variation
学科领域Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
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收录类别sci
资助项目LMB
WOS记录号WOS:000279303700011
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/3800
专题中科院海洋生物资源可持续利用重点实验室
通讯作者chen.chang@scsio.ac.cn; cqhu@scsio.ac.cn
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Chen, C,Zhao, JJ,Ren, CH,et al. Deletion of valR, a homolog of Vibrio harveyis luxR generates an intermediate colony phenotype between opaque/rugose and translucent/smooth in Vibrio alginolyticus[J]. BIOFOULING,2010,26(5):595-601.
APA Chen, C,Zhao, JJ,Ren, CH,Hu, CQ,chen.chang@scsio.ac.cn,&cqhu@scsio.ac.cn.(2010).Deletion of valR, a homolog of Vibrio harveyis luxR generates an intermediate colony phenotype between opaque/rugose and translucent/smooth in Vibrio alginolyticus.BIOFOULING,26(5),595-601.
MLA Chen, C,et al."Deletion of valR, a homolog of Vibrio harveyis luxR generates an intermediate colony phenotype between opaque/rugose and translucent/smooth in Vibrio alginolyticus".BIOFOULING 26.5(2010):595-601.
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