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学科主题: Biotechnology & Applied Microbiology; Marine & Freshwater Biology
题名: Deletion of valR, a homolog of Vibrio harveyis luxR generates an intermediate colony phenotype between opaque/rugose and translucent/smooth in Vibrio alginolyticus
作者: Chen, C ; Zhao, JJ ; Ren, CH ; Hu, CQ
通讯作者: chen.chang@scsio.ac.cn ; cqhu@scsio.ac.cn
关键词: Vibrio alginolyticus ; biofilm formation ; quorum sensing ; colony phase variation
刊名: BIOFOULING
发表日期: 2010
卷: 26, 期:5, 页:595-601
收录类别: sci
部门归属: [Chen Chang] CAS, LMB, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; CAS, S China Sea Inst Oceanol, LAMB, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
摘要: A previous study has shown that Vibrio alginolyticus ZJ-51 undergoes colony phase variation between opaque/ rugose (Op) and translucent/smooth (Tr). The AI-2 quorum-sensing master regulator ValR, a homolog to V. harveyi LuxR, was suggested to be involved in the transition. To investigate the role of ValR in the variation and in biofilm formation, an in-frame deletion of valR in both Op and Tr backgrounds was carried out. The mutants in both backgrounds showed an intermediate colony morphotype, where the colonies were less opaque/rugose but not fully translucent/smooth either. They also showed an intermediate level of motility. However, biofilm formation was severely decreased in both mutants and polar flagella were depleted also. Quantitative PCR showed that most of the genes related to flagellar and polysaccharide biosynthesis were upregulated in the mutant of Op background (DvalR/Op) but downregulated in the mutant of Tr background (DvalR/Tr) compared with their parental wild-type strains. This suggests that ValR may control biofilm formation by regulating flagellar biosynthesis and affect the expression of the genes involved in colony phase variation in V. alginolyticus.
原文出处: 查看原文
WOS记录号: WOS:000279303700011
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/3800
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Chen, C; Zhao, JJ; Ren, CH; Hu, CQ.Deletion of valR, a homolog of Vibrio harveyis luxR generates an intermediate colony phenotype between opaque/rugose and translucent/smooth in Vibrio alginolyticus,BIOFOULING,2010,26(5):595-601
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