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学科主题: Virology
题名: Nuclear-export-signal-dependent protein translocation of dUTPase encoded by Singapore grouper iridovirus
作者: Gong, J ; Huang, YH ; Huang, XH ; Zhang, R ; Qin, QW
通讯作者: qinqw@scsio.ac.cn
刊名: ARCHIVES OF VIROLOGY
发表日期: 2010
卷: 155, 期:7, 页:1069-1076
收录类别: sci
部门归属: [Huang, Xiao-Hong; Qin, Qi-Wei] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Gong, Jie; Huang, You-Hua; Zhang, Rong] Sun Yat Sen Zhongshan Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China
项目归属: LMB
摘要: The dUTPase is a ubiquitous and crucial enzyme responsible for regulating cellular levels of dUTP. In the present study, the expression pattern and translocation of a dUTPase homolog encoded by Singapore grouper iridovirus (SGIV) were elucidated. The SGIV ORF049R encodes a dUTPase homolog, which is a peptide of 155 amino acids that contains five conserved motifs. The temporal expression pattern during infection in vitro revealed that the SGIV dUTPase was an early transcript. A leucine-rich nuclear export signal (NES) at the C-terminus was predicted using CBS Online Servers. Subcellular location analysis showed that SGIV dUTPase is a cytoplasmic protein. Site-direct mutagenesis by overlap extension-PCR indicated that the NES is crucial for the translocation of SGIV dUTPase from the nucleus to the cytoplasm. We have discovered for the first time that the NES-dependent translocation of dUTPase is different for SGIV than for members of other species, which depend on a nuclear localization signal. These results provide new insights into the pathogenesis of fish iridoviruses.
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WOS记录号: WOS:000279453200006
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/3802
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Gong, J; Huang, YH; Huang, XH; Zhang, R; Qin, QW.Nuclear-export-signal-dependent protein translocation of dUTPase encoded by Singapore grouper iridovirus,ARCHIVES OF VIROLOGY,2010,155(7):1069-1076
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文件名: Nuclear-export-signal-dependent protein translocation of dUTPase encoded by Singapore grouper iridovirus .pdf
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