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学科主题: Agriculture, Dairy & Animal Science; Food Science & Technology
题名: The phenotypic and genotypic characterization of Enterobacter sakazakii strains from infant formula milk
作者: Ye, Y ; Wu, Q ; Xu, X ; Yang, X ; Dong, X ; Zhang, J
通讯作者: yeyw04@mails.gucas.ac.cn ; wuqp203@yahoo.com.cn
关键词: Enterobacter sakazakii ; enterobacterial repetitive intergenic consensus (ERIC)-PCR ; random amplified polymorphic DNA (RAPD)-PCR ; antibiotic resistance test
刊名: JOURNAL OF DAIRY SCIENCE
发表日期: 2010
卷: 93, 期:6, 页:2315-2320
收录类别: sci
部门归属: [Ye, Y.; Wu, Q.; Xu, X.; Yang, X.; Dong, X.; Zhang, J.] Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbiol Culture Collect, Guangzhou 510070, Guangdong, Peoples R China; [Ye, Y.; Dong, X.] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Ye, Y.] Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Peoples R China
项目归属: LMB
摘要: Enterobacter sakazakii is an emerging foodborne pathogen associated with severe diseases in neonates. Infant formula milk (IFM) has been identified as one of the major contaminated sources and a transmission vehicle. To determine the phenotypic and genotypic characterization of this pathogen, 22 E. sakazakii strains isolated from IFM by an FDA-recommended method and PCR on the a-glucosidase gene were subtyped by random amplified polymorphic DNA (RAPD)-PCR, enterobacterial repetitive intergenic consensus (ERIC)-PCR, and antibiotic resistance patterns. At a similarity threshold of 80%, 16 ERIC-PCR fingerprint types were identified with a discriminatory power (D) of 0.933, and 18 RAPD-PCR types were identified with D of 0.973. Resistance to 9 antibiotics tested by disk diffusion assay revealed 6 antibiotic resistance patterns with D of 0.749. The comparison of characterization indicated that RAPD-PCR and ERIC-PCR have high discriminatory power showing genetic diversity of E. sakazakii isolates, and ERIC-PCR patterns showed a closer correlation than RAPD-PCR patterns to phenotypic characterization and the brands of IFM. Overall, the ERIC-PCR typing method could be used for tracing sources of E. sakazakii isolates in the food chain.
原文出处: 查看原文
WOS记录号: WOS:000277929100002
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/3836
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Ye, Y; Wu, Q; Xu, X; Yang, X; Dong, X; Zhang, J.The phenotypic and genotypic characterization of Enterobacter sakazakii strains from infant formula milk,JOURNAL OF DAIRY SCIENCE,2010,93(6):2315-2320
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