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学科主题: Microbiology
题名: Culture-dependent and culture-independent diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve from the South China Sea
作者: Sun, W ; Dai, SK ; Jiang, SM ; Wang, GH ; Liu, GH ; Wu, HB ; Li, X
通讯作者: wuhoubo@scsio.ac.cn ; lixiang@scsio.ac.cn
关键词: Actinobacteria ; Diversity ; Marine sponge ; Salinispora
刊名: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
发表日期: 2010
卷: 98, 期:1, 页:65-75
收录类别: sci
部门归属: [Sun, Wei; Dai, Shikun; Jiang, Shumei; Wang, Guanghua; Liu, Guohui; Wu, Houbo; Li, Xiang] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, Guangdong Key Lab Marine Mat Med LMMM GD, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Sun, Wei; Dai, Shikun; Liu, Guohui] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
项目归属: LMB
摘要: In this report, the diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve collected from a remote island of the South China Sea was investigated employing classical cultivation and characterization, 16S rDNA library construction, 16S rDNA-restriction fragment length polymorphism (rDNA-RFLP) and phylogenetic analysis. A total of 184 strains were isolated using seven different media and 24 isolates were selected according to their morphological characteristics for phylogenetic analysis on the basis of their 16S rRNA gene sequences. Results showed that the 24 isolates were assigned to six genera including Salinispora, Gordonia, Mycobacterium, Nocardia, Rhodococcus and Streptomyces. This is the first report that Salinispora is present in a marine sponge from the South China Sea. Subsequently, 26 rDNA clones were selected from 191 clones in an Actinobacteria-specific 16S rDNA library of the H. perleve sample, using the RFLP technique for sequencing and phylogenetic analysis. In total, 26 phylotypes were clustered in eight known genera of Actinobacteria including Mycobacterium, Amycolatopsis, Arthrobacter, Brevibacterium, Microlunatus, Nocardioides, Pseudonocardia and Streptomyces. This study contributes to our understanding of actinobacterial diversity in the marine sponge H. perleve from the South China Sea.
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WOS记录号: WOS:000276902800007
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/3850
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Sun, W; Dai, SK; Jiang, SM; Wang, GH; Liu, GH; Wu, HB; Li, X.Culture-dependent and culture-independent diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve from the South China Sea,ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2010,98(1):65-75
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