Identification and characterization of Singapore grouper iridovirus (SGIV) ORF162L, an immediate-early gene involved in cell growth control and viral replication
Xia, LQ; Liang, HY; Huang, YH; Ou-Yang, ZL; Qin, QW; qinqw@scsio.ac.cn
2010
发表期刊VIRUS RESEARCH
ISSN0168-1702
卷号147期号:1页码:30-39
摘要Singapore grouper iridovirus (SGIV) is a major pathogen resulting in heavy economic losses to grouper aquaculture. In this study, SGIV ORF162L encoding a putative homolog of ICP46 was identified and characterized. Interestingly, ICP46 could be found in all sequenced iridoviruses and is considered as a core gene of the family Iridoviridae. SGIV ICP46 was classified as an immediate-early (IE) gene during in vitro infection using drug inhibition analysis, reverse transcription polymerase chain reaction and Western blot analysis. Subcellular localization revealed that SGIV ICP46 was distributed predominantly in the cytoplasm. Furthermore, SGIV ICP46 proved to be a structural protein of the nucleocapsid; its overexpression could promote the growth of grouper embryonic cells and contribute to SGIV replication. This is the first report of the characterization of a putative ICP46 homolog and these results should offer important insights into the pathogenesis of iridoviruses. (c) 2009 Elsevier B.V. All rights reserved.
部门归属[Qin, Qiwei] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresourses Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Xia, Liqun; Liang, Haiying] Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524025, Guangdong, Peoples R China; [Huang, Youhua; Ou-Yang, Zhengliang] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China
关键词Iridovirus Sgiv Icp46 Structural Protein Cell Growth Viral Replication
学科领域Virology
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收录类别sci
资助项目LMB
WOS记录号WOS:000276560300005
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/3858
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
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Xia, LQ,Liang, HY,Huang, YH,et al. Identification and characterization of Singapore grouper iridovirus (SGIV) ORF162L, an immediate-early gene involved in cell growth control and viral replication[J]. VIRUS RESEARCH,2010,147(1):30-39.
APA Xia, LQ,Liang, HY,Huang, YH,Ou-Yang, ZL,Qin, QW,&qinqw@scsio.ac.cn.(2010).Identification and characterization of Singapore grouper iridovirus (SGIV) ORF162L, an immediate-early gene involved in cell growth control and viral replication.VIRUS RESEARCH,147(1),30-39.
MLA Xia, LQ,et al."Identification and characterization of Singapore grouper iridovirus (SGIV) ORF162L, an immediate-early gene involved in cell growth control and viral replication".VIRUS RESEARCH 147.1(2010):30-39.
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