[Li, Chenghua; Chen, Aiqin; Wu, Huifeng; Qin, Song; Xue, Qinzhao; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Sun, Huili] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Ning, Xuanxuan] SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
项目归属:
LMB
摘要:
Superoxide dismutase (SOD, EC 115.11) represents one kind of enzyme involved in scavenging the high level of reactive oxygen species (ROS) into molecular oxygen and hydrogen peroxide In the present study, the intracellular Cu/Zn-SOD gene (icCu/Zn-SOD) of Venerupis philippinarum (denoted as VpSOD) was identified from haemocytes by homology cloning and RACR approaches The full-length cDNA of VpSOD consisted of 910 nucleotides with a canonical polyadenylation signal sequence AATAAA, a polyA tail, and an open-reading frame of 465 bp encoding 154 amino acids The deduced amino acid of VpSOD shared high similarity with the icCu/Zn-SODs from other species, indicating that VpSOD should be a new member of icCu/Zn-SOD family Several highly conserved motifs including Cu, Zn binding sites (H-46, H-48, H-63, H-120 for Cu binding, and H-63, H-71, H-80, D-83 for Zn binding). intracellular disulfide bond and two Cu, Zn SOD signatures were also identified in VpSOD The temporal expression of VpSOD in haemocytes after Vibrio anguillarum challenge was recorded by quantitative real-time RT-PCR. The relative expression level of VpSOD mRNA Was Lip-regulated rapidly at 6 h post-infection and reached 18-fold of the control group. After a drastic decrease at 12 h. the expression level increased again and reached 22-fold to that in the control group at 96 h post-infection. All these results indicated that VpSOD was an acute-phase protein involved in the immune responses of V philippinarum. (C) 2009 Elsevier Ltd. All rights reserved
