Identification and characterization of a novel capsid protein encoded by Singapore grouper iridovirus ORF038L
Wan, QJ; Gong, J; Huang, XH; Huang, YH; Zhou, S; Ou-Yang, ZL; Cao, JH; Ye, LL; Qin, QW; qinqw@scsio.ac.cn
2010
发表期刊ARCHIVES OF VIROLOGY
ISSN0304-8608
卷号155期号:3页码:351-359
摘要Singapore grouper iridovirus (SGIV) is an important pathogen isolated from grouper, Epinephelus tauvina, and characterized as a novel ranavirus. To better understand the function of viral structural genes involved in SGIV infection and virus-host interactions, a candidate gene, VP38 (ORF038L), was investigated in this study. SGIV VP38 was found to encode a 170-aa peptide containing an RGD motif, and it showed significant identity only to members of the genus Iridovirus, family Iridoviridae, except megalocytivirus. The VP38 gene was identified by temporal expression pattern analysis and drug inhibition assay as a late (L) gene. Immunofluorescence localization revealed that P38 was distributed predominately in the cytoplasm and that association of VP38 with viral factories increased at the late stage of SGIV infection. Consistent results from immunoelectron microscopy (IEM) and western blot analysis revealed that SGIV VP38 is a viral capsid protein. Furthermore, antibodies specific for SGIV VP38 exhibited substantial SGIV-neutralizing activity in vitro, suggesting that VP38 might play an important role in SGIV infectivity.
部门归属[Huang, Xiao Hong; Qin, Qi Wei] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Wan, Qing Jiao; Gong, Jie; Huang, You Hua; Zhou, Sheng; Ou-Yang, Zheng Liang; Cao, Jian Hao; Ye, Li Li] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China
学科领域Virology
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收录类别sci
资助项目LMB
WOS记录号WOS:000274939600007
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被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/3900
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
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Wan, QJ,Gong, J,Huang, XH,et al. Identification and characterization of a novel capsid protein encoded by Singapore grouper iridovirus ORF038L[J]. ARCHIVES OF VIROLOGY,2010,155(3):351-359.
APA Wan, QJ.,Gong, J.,Huang, XH.,Huang, YH.,Zhou, S.,...&qinqw@scsio.ac.cn.(2010).Identification and characterization of a novel capsid protein encoded by Singapore grouper iridovirus ORF038L.ARCHIVES OF VIROLOGY,155(3),351-359.
MLA Wan, QJ,et al."Identification and characterization of a novel capsid protein encoded by Singapore grouper iridovirus ORF038L".ARCHIVES OF VIROLOGY 155.3(2010):351-359.
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