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学科主题: Virology
题名: Identification and characterization of a novel capsid protein encoded by Singapore grouper iridovirus ORF038L
作者: Wan, QJ ; Gong, J ; Huang, XH ; Huang, YH ; Zhou, S ; Ou-Yang, ZL ; Cao, JH ; Ye, LL ; Qin, QW
通讯作者: qinqw@scsio.ac.cn
刊名: ARCHIVES OF VIROLOGY
发表日期: 2010
卷: 155, 期:3, 页:351-359
收录类别: sci
部门归属: [Huang, Xiao Hong; Qin, Qi Wei] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Wan, Qing Jiao; Gong, Jie; Huang, You Hua; Zhou, Sheng; Ou-Yang, Zheng Liang; Cao, Jian Hao; Ye, Li Li] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China
项目归属: LMB
摘要: Singapore grouper iridovirus (SGIV) is an important pathogen isolated from grouper, Epinephelus tauvina, and characterized as a novel ranavirus. To better understand the function of viral structural genes involved in SGIV infection and virus-host interactions, a candidate gene, VP38 (ORF038L), was investigated in this study. SGIV VP38 was found to encode a 170-aa peptide containing an RGD motif, and it showed significant identity only to members of the genus Iridovirus, family Iridoviridae, except megalocytivirus. The VP38 gene was identified by temporal expression pattern analysis and drug inhibition assay as a late (L) gene. Immunofluorescence localization revealed that P38 was distributed predominately in the cytoplasm and that association of VP38 with viral factories increased at the late stage of SGIV infection. Consistent results from immunoelectron microscopy (IEM) and western blot analysis revealed that SGIV VP38 is a viral capsid protein. Furthermore, antibodies specific for SGIV VP38 exhibited substantial SGIV-neutralizing activity in vitro, suggesting that VP38 might play an important role in SGIV infectivity.
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WOS记录号: WOS:000274939600007
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/3900
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Wan, QJ; Gong, J; Huang, XH; Huang, YH; Zhou, S; Ou-Yang, ZL; Cao, JH; Ye, LL; Qin, QW.Identification and characterization of a novel capsid protein encoded by Singapore grouper iridovirus ORF038L,ARCHIVES OF VIROLOGY,2010,155(3):351-359
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文件名: Identification and characterization of a novel capsid protein encoded by Singapore grouper iridovirus ORF038L .pdf
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