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学科主题: Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
题名: Molecular cloning, characterization and expression analysis of a C-type lectin (Ec-CTL) in orange-spotted grouper, Epinephelus coioides
作者: Wei, JG ; Xu, D ; Zhou, JG ; Cui, HC ; Yan, Y ; Ouyang, ZL ; Gong, J ; Huang, YH ; Huang, XH ; Qjn, QW
通讯作者: qinqw@scsio.ac.cn
关键词: Epinephelus coioides ; Singapore grouper iridovirus (SGIV) ; C-type lectin ; Molecular cloning ; Expression
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2010
卷: 28, 期:1, 页:178-186
收录类别: sci
部门归属: [Huang, Xiaohong; Qjn, Qiwei] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Wei, Jingguang; Xu, Dan; Zhou, Jinggeng; Cui, Huachun; Yan, Yang; Ouyang, Zhengliang; Gong, Jie; Huang, Youhua] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China
项目归属: LMB
摘要: C-type lectins play crucial roles in pathogen recognition, innate immunity, and cell-cell interactions. In this study, a new C-type lectin (Ec-CTL) gene was cloned from grouper, Epinephelus coioides by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of Ec-CTL was composed of 840 bp with a 651 bp open reading frame (ORF) that encodes a 216-residue protein. The deduced amino acid sequence of Ec-CTL possessed all conserved features crucial for the fundamental structure, such as the four cysteine residues (Cys(71), Cys(152), Cys(167), Cys(175)) involved in the formation of disulphide bridges and the potential Ca2+/carbohydrate-binding sites. Ec-CTL contains a signal peptide and a single carbohydrate recognition domain (CRD). The genomic DNA of the gene consists of three exons and two introns. Ec-CTL showed high similarity of 54% to the C-type lectin of killifish Fundulus heteroclitus. Ec-CTL mRNA is predominately expressed in liver and skin, and lower expressed in kidney, intestine, heart, brain and spleen. The expression of Ec-CTL was differentially up-regulated in orange-spotted grouper challenged with Saccharomyces cerevisiae, Vibrio vulnificus, Staphyloccocus aureus and Singapore grouper iridovirus (SGIV). Recombinant mature Ec-CTL (rEc-CTL) was expressed in E. coli BL21, purified and characterized as a typical Ca2+-dependent carbohydrate-binding protein possessing hemagglutinating activity. It bound to all examined bacterial and yeast strains, and aggregated with S. cerevisiae, V vulnificus and S. aureus in a Ca2+-dependent manner. (C) 2009 Elsevier Ltd. All rights reserved.
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WOS记录号: WOS:000274235100022
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/3904
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Wei, JG; Xu, D; Zhou, JG; Cui, HC; Yan, Y; Ouyang, ZL; Gong, J; Huang, YH; Huang, XH; Qjn, QW.Molecular cloning, characterization and expression analysis of a C-type lectin (Ec-CTL) in orange-spotted grouper, Epinephelus coioides,FISH & SHELLFISH IMMUNOLOGY,2010,28(1):178-186
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文件名: Molecular cloning, characterization and expression analysis of a C-type lectin (Ec-CTL) in orange-spotted grouper, Epinephelus coioides .pdf
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