Molecular cloning, characterization and expression analysis of a C-type lectin (Ec-CTL) in orange-spotted grouper, Epinephelus coioides
Wei, JG; Xu, D; Zhou, JG; Cui, HC; Yan, Y; Ouyang, ZL; Gong, J; Huang, YH; Huang, XH; Qjn, QW; qinqw@scsio.ac.cn
2010
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号28期号:1页码:178-186
摘要C-type lectins play crucial roles in pathogen recognition, innate immunity, and cell-cell interactions. In this study, a new C-type lectin (Ec-CTL) gene was cloned from grouper, Epinephelus coioides by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of Ec-CTL was composed of 840 bp with a 651 bp open reading frame (ORF) that encodes a 216-residue protein. The deduced amino acid sequence of Ec-CTL possessed all conserved features crucial for the fundamental structure, such as the four cysteine residues (Cys(71), Cys(152), Cys(167), Cys(175)) involved in the formation of disulphide bridges and the potential Ca2+/carbohydrate-binding sites. Ec-CTL contains a signal peptide and a single carbohydrate recognition domain (CRD). The genomic DNA of the gene consists of three exons and two introns. Ec-CTL showed high similarity of 54% to the C-type lectin of killifish Fundulus heteroclitus. Ec-CTL mRNA is predominately expressed in liver and skin, and lower expressed in kidney, intestine, heart, brain and spleen. The expression of Ec-CTL was differentially up-regulated in orange-spotted grouper challenged with Saccharomyces cerevisiae, Vibrio vulnificus, Staphyloccocus aureus and Singapore grouper iridovirus (SGIV). Recombinant mature Ec-CTL (rEc-CTL) was expressed in E. coli BL21, purified and characterized as a typical Ca2+-dependent carbohydrate-binding protein possessing hemagglutinating activity. It bound to all examined bacterial and yeast strains, and aggregated with S. cerevisiae, V vulnificus and S. aureus in a Ca2+-dependent manner. (C) 2009 Elsevier Ltd. All rights reserved.
部门归属[Huang, Xiaohong; Qjn, Qiwei] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Wei, Jingguang; Xu, Dan; Zhou, Jinggeng; Cui, Huachun; Yan, Yang; Ouyang, Zhengliang; Gong, Jie; Huang, Youhua] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China
关键词Epinephelus Coioides Singapore Grouper Iridovirus (Sgiv) C-type Lectin Molecular Cloning Expression
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
URL查看原文
收录类别sci
资助项目LMB
WOS记录号WOS:000274235100022
引用统计
被引频次:38[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/3904
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
推荐引用方式
GB/T 7714
Wei, JG,Xu, D,Zhou, JG,et al. Molecular cloning, characterization and expression analysis of a C-type lectin (Ec-CTL) in orange-spotted grouper, Epinephelus coioides[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(1):178-186.
APA Wei, JG.,Xu, D.,Zhou, JG.,Cui, HC.,Yan, Y.,...&qinqw@scsio.ac.cn.(2010).Molecular cloning, characterization and expression analysis of a C-type lectin (Ec-CTL) in orange-spotted grouper, Epinephelus coioides.FISH & SHELLFISH IMMUNOLOGY,28(1),178-186.
MLA Wei, JG,et al."Molecular cloning, characterization and expression analysis of a C-type lectin (Ec-CTL) in orange-spotted grouper, Epinephelus coioides".FISH & SHELLFISH IMMUNOLOGY 28.1(2010):178-186.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Molecular cloning, c(1248KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。