Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization
Shi, Y; Zhao, Z; Yin, JK; Zhu, XP; Chen, KC; Pan, DB; Gui, JF; zhuxinping_1964@yahoo.com.cn
2010
发表期刊COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
卷号155期号:2页码:110-117
摘要Interferon regulatory factor 2 (IRF-2) is a multifunctional transcription factor which exhibits both transcriptional activating and repressing activities in the IRF family. In this study, we report an IRF-2 gene isolated from orange-spotted grouper (Epinephelus coioides). The 1854 bp full-length cDNA sequence of EcIRF-2 has been cloned, encoding a putative peptide of 336 amino acids which is highly consistent with the feature of IRF family members. The genomic fragment of EcIRF-2 contains nine exons and eight introns, spanning over approximate 8.8 kb. The expression of EcIRF-2 gene was detected in various tissues of healthy orange-spotted grouper and in four tissues after being challenged with poly I:C or LPS. EcIRF-2 gene is ubiquitously expressed in various healthy fish tissues and is up-regulated in vivo in response to poly I:C or LPS. Subcellular localization analysis of EcIRF-2 suggests it is an intranuclear protein in the fish cells. We believe this research is the first report of fish IRF-2 protein localization. The results in this research establish the base for further study of function mechanism of IRF family members in orange-spotted grouper. (C) 2009 Elsevier Inc. All rights reserved.
部门归属[Shi, Yan; Yin, Jing-Kui; Zhu, Xin-Ping; Chen, Kun-Ci; Pan, De-Bo] Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China; [Zhao, Zhe] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Zhu, Xin-Ping] Guangzhou Univ, Coll Life Sci, Guangzhou 510006, Guangdong, Peoples R China; [Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词Interferon Regulator Factor-2 (Irf-2) Epinephelus Coioides Genomic Structure Inductive Expression Subcellular Localization
学科领域Biochemistry & Molecular Biology ; Zoology
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收录类别sci
资助项目LMB
WOS记录号WOS:000273927000003
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被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/3912
专题中科院海洋生物资源可持续利用重点实验室
通讯作者zhuxinping_1964@yahoo.com.cn
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Shi, Y,Zhao, Z,Yin, JK,et al. Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2010,155(2):110-117.
APA Shi, Y.,Zhao, Z.,Yin, JK.,Zhu, XP.,Chen, KC.,...&zhuxinping_1964@yahoo.com.cn.(2010).Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,155(2),110-117.
MLA Shi, Y,et al."Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 155.2(2010):110-117.
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