中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 海洋生物  > 期刊论文
学科主题: Fisheries; Veterinary Sciences
题名: Purification and antigenic characteristics of a rickettsia-like organism from the oyster Crassostrea ariakensis
作者: Wu, XZ ; Sun, JF ; Zhang, WZ ; Wen, BB
通讯作者: wuxz@zju.edu.cn
关键词: rickettsia-like organism ; Crassostrea ariakensis ; antigenic characteristics ; purification
刊名: DISEASES OF AQUATIC ORGANISMS
发表日期: 2005
卷: 67, 期:40545, 页:149-154
收录类别: sci
部门归属: Zhejiang Univ, Coll Anim Sci, Hangzhou 310029, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Peoples R China; Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China
摘要: A rickettsia-like organism (RLO) has been suggested to be the etiological agent responsible for heavy losses of the oyster Crassostrea ariakensis Gould in China. Because of the lack of molluscan cell lines for in vitro culture of intracellular prokaryotes, antigenic analysis of RLOs has been limited by the inherent difficulties of their purification. In this report, we describe the use of differential speed centrifugation and renografin density gradient centrifugation to purify the RLO directly from infected oyster tissues. The purity and integrity of purified prokaryotes were validated by transmission electron microscopy. Thirteen major constituent proteins, with molecular weights ranging between 17 and 99 kDa, were electrophoretically identified by silver staining, and 8 major proteins were identified with Coomassie blue R staining. Specific mouse polyclonal antiserum was prepared for serological characterization of the RLO and was used in an immunoblot assay, and 3 major antigen groups were identified. The present results advance our knowledge of RLO protein antigens, and several proteins have been identified that could potentially be useful for diagnostic assays or for production of experimental immunostimulants.
WOS记录号: WOS:000234935200021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/3946
Appears in Collections:海洋生物_期刊论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Wu, XZ; Sun, JF; Zhang, WZ; Wen, BB.Purification and antigenic characteristics of a rickettsia-like organism from the oyster Crassostrea ariakensis,DISEASES OF AQUATIC ORGANISMS,2005,67(40545):149-154
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wu, XZ]'s Articles
[Sun, JF]'s Articles
[Zhang, WZ]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wu, XZ]‘s Articles
[Sun, JF]‘s Articles
[Zhang, WZ]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace