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Purification and antigenic characteristics of a rickettsia-like organism from the oyster Crassostrea ariakensis
Wu, XZ; Sun, JF; Zhang, WZ; Wen, BB; wuxz@zju.edu.cn
2005
发表期刊DISEASES OF AQUATIC ORGANISMS
ISSN0177-5103
卷号67期号:40545页码:149-154
摘要A rickettsia-like organism (RLO) has been suggested to be the etiological agent responsible for heavy losses of the oyster Crassostrea ariakensis Gould in China. Because of the lack of molluscan cell lines for in vitro culture of intracellular prokaryotes, antigenic analysis of RLOs has been limited by the inherent difficulties of their purification. In this report, we describe the use of differential speed centrifugation and renografin density gradient centrifugation to purify the RLO directly from infected oyster tissues. The purity and integrity of purified prokaryotes were validated by transmission electron microscopy. Thirteen major constituent proteins, with molecular weights ranging between 17 and 99 kDa, were electrophoretically identified by silver staining, and 8 major proteins were identified with Coomassie blue R staining. Specific mouse polyclonal antiserum was prepared for serological characterization of the RLO and was used in an immunoblot assay, and 3 major antigen groups were identified. The present results advance our knowledge of RLO protein antigens, and several proteins have been identified that could potentially be useful for diagnostic assays or for production of experimental immunostimulants.
部门归属Zhejiang Univ, Coll Anim Sci, Hangzhou 310029, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Peoples R China; Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China
关键词Rickettsia-like Organism Crassostrea Ariakensis Antigenic Characteristics Purification
学科领域Fisheries ; Veterinary Sciences
收录类别sci
WOS记录号WOS:000234935200021
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/3946
专题海洋生物
通讯作者wuxz@zju.edu.cn
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Wu, XZ,Sun, JF,Zhang, WZ,et al. Purification and antigenic characteristics of a rickettsia-like organism from the oyster Crassostrea ariakensis[J]. DISEASES OF AQUATIC ORGANISMS,2005,67(40545):149-154.
APA Wu, XZ,Sun, JF,Zhang, WZ,Wen, BB,&wuxz@zju.edu.cn.(2005).Purification and antigenic characteristics of a rickettsia-like organism from the oyster Crassostrea ariakensis.DISEASES OF AQUATIC ORGANISMS,67(40545),149-154.
MLA Wu, XZ,et al."Purification and antigenic characteristics of a rickettsia-like organism from the oyster Crassostrea ariakensis".DISEASES OF AQUATIC ORGANISMS 67.40545(2005):149-154.
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