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学科主题: Food Science & Technology
题名: The application of PCR-RFLP and FINS for species identification used in sea cucumbers (Aspidochirotida: Stichopodidae) products from the market
作者: Wen, J ; Hu, CQ ; Zhang, L ; Luo, P ; Zhao, Z ; Fan, SG ; Su, T
通讯作者: cqhu@scsio.ac.cn
关键词: Sea cucumber ; PCR-RFLP ; FINS ; 16S rRNA gene ; Species identification
刊名: FOOD CONTROL
发表日期: 2010
卷: 21, 期:4, 页:403-407
收录类别: sci
部门归属: [Wen, Jing; Hu, Chaoqun; Zhang, Lvping; Luo, Peng; Zhao, Zhe; Fan, Sigang; Su, Ting] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China; [Wen, Jing; Hu, Chaoqun; Zhang, Lvping; Luo, Peng; Zhao, Zhe; Fan, Sigang; Su, Ting] Chinese Acad Sci, Chinese Acad Sci LAMB, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Wen, Jing; Fan, Sigang; Su, Ting] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
项目归属: 海洋地质
摘要: Sea cucumbers are traditional, delicious and luxurious seafood in Asia. They are usually provided to the consumers in the marketing formats of dried or frozen products. At the commercial level, they are much different not only in flavor. but also in price. The processing treatment makes them difficult for species identification based on morphological characterization. In this study, two molecular methods are developed to identify six species belonging to family Stichopodidae in commercial products. The first one is PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism), which uses PCR amplification of a common 570 bp fragment of the 16S rRNA gene and follows codigestion with two restriction enzymes (Xmnl and Mwol). Another one is FINS (forensically informative nucleotide sequencing) of the 16S rRNA gene, and the key of the method is the phylogenetic analysis of 16S rRNA gene sequences. The PCR-RFLP methodology can be used to authenticate the species tested in the present study and allow us to detect a fraudulent labeling of frozen Apostichopus japonicus product. And the FINS methodology confirms that the species substituted for A. japonicus belongs to the genera Isostichopus. Therefore, two molecular tools really provide convenient, useful and academic techniques to authenticate the species of commercial sea cucumber products. (C) 2009 Elsevier Ltd. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000272752800008
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/4354
Appears in Collections:中科院边缘海地质重点实验室_期刊论文

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Wen, J; Hu, CQ; Zhang, L; Luo, P; Zhao, Z; Fan, SG; Su, T.The application of PCR-RFLP and FINS for species identification used in sea cucumbers (Aspidochirotida: Stichopodidae) products from the market,FOOD CONTROL,2010,21(4):403-407
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