SCSIO OpenIR  > 中科院边缘海地质重点实验室
The application of PCR-RFLP and FINS for species identification used in sea cucumbers (Aspidochirotida: Stichopodidae) products from the market
Wen, J; Hu, CQ; Zhang, L; Luo, P; Zhao, Z; Fan, SG; Su, T; cqhu@scsio.ac.cn
2010
发表期刊FOOD CONTROL
ISSN0956-7135
卷号21期号:4页码:403-407
摘要Sea cucumbers are traditional, delicious and luxurious seafood in Asia. They are usually provided to the consumers in the marketing formats of dried or frozen products. At the commercial level, they are much different not only in flavor. but also in price. The processing treatment makes them difficult for species identification based on morphological characterization. In this study, two molecular methods are developed to identify six species belonging to family Stichopodidae in commercial products. The first one is PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism), which uses PCR amplification of a common 570 bp fragment of the 16S rRNA gene and follows codigestion with two restriction enzymes (Xmnl and Mwol). Another one is FINS (forensically informative nucleotide sequencing) of the 16S rRNA gene, and the key of the method is the phylogenetic analysis of 16S rRNA gene sequences. The PCR-RFLP methodology can be used to authenticate the species tested in the present study and allow us to detect a fraudulent labeling of frozen Apostichopus japonicus product. And the FINS methodology confirms that the species substituted for A. japonicus belongs to the genera Isostichopus. Therefore, two molecular tools really provide convenient, useful and academic techniques to authenticate the species of commercial sea cucumber products. (C) 2009 Elsevier Ltd. All rights reserved.
部门归属[Wen, Jing; Hu, Chaoqun; Zhang, Lvping; Luo, Peng; Zhao, Zhe; Fan, Sigang; Su, Ting] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China; [Wen, Jing; Hu, Chaoqun; Zhang, Lvping; Luo, Peng; Zhao, Zhe; Fan, Sigang; Su, Ting] Chinese Acad Sci, Chinese Acad Sci LAMB, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Wen, Jing; Fan, Sigang; Su, Ting] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
关键词Sea Cucumber Pcr-rflp Fins 16s Rrna Gene Species Identification
学科领域Food Science & Technology
URL查看原文
收录类别sci
资助项目海洋地质
WOS记录号WOS:000272752800008
引用统计
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/4354
专题中科院边缘海地质重点实验室
通讯作者cqhu@scsio.ac.cn
推荐引用方式
GB/T 7714
Wen, J,Hu, CQ,Zhang, L,et al. The application of PCR-RFLP and FINS for species identification used in sea cucumbers (Aspidochirotida: Stichopodidae) products from the market[J]. FOOD CONTROL,2010,21(4):403-407.
APA Wen, J.,Hu, CQ.,Zhang, L.,Luo, P.,Zhao, Z.,...&cqhu@scsio.ac.cn.(2010).The application of PCR-RFLP and FINS for species identification used in sea cucumbers (Aspidochirotida: Stichopodidae) products from the market.FOOD CONTROL,21(4),403-407.
MLA Wen, J,et al."The application of PCR-RFLP and FINS for species identification used in sea cucumbers (Aspidochirotida: Stichopodidae) products from the market".FOOD CONTROL 21.4(2010):403-407.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
The application of P(244KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。