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The application of PCR-RFLP and FINS for species identification used in sea cucumbers (Aspidochirotida: Stichopodidae) products from the market
Wen, J; Hu, CQ; Zhang, L; Luo, P; Zhao, Z; Fan, SG; Su, T; cqhu@scsio.ac.cn
2010
Source PublicationFOOD CONTROL
ISSN0956-7135
Volume21Issue:4Pages:403-407
AbstractSea cucumbers are traditional, delicious and luxurious seafood in Asia. They are usually provided to the consumers in the marketing formats of dried or frozen products. At the commercial level, they are much different not only in flavor. but also in price. The processing treatment makes them difficult for species identification based on morphological characterization. In this study, two molecular methods are developed to identify six species belonging to family Stichopodidae in commercial products. The first one is PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism), which uses PCR amplification of a common 570 bp fragment of the 16S rRNA gene and follows codigestion with two restriction enzymes (Xmnl and Mwol). Another one is FINS (forensically informative nucleotide sequencing) of the 16S rRNA gene, and the key of the method is the phylogenetic analysis of 16S rRNA gene sequences. The PCR-RFLP methodology can be used to authenticate the species tested in the present study and allow us to detect a fraudulent labeling of frozen Apostichopus japonicus product. And the FINS methodology confirms that the species substituted for A. japonicus belongs to the genera Isostichopus. Therefore, two molecular tools really provide convenient, useful and academic techniques to authenticate the species of commercial sea cucumber products. (C) 2009 Elsevier Ltd. All rights reserved.
Department[Wen, Jing; Hu, Chaoqun; Zhang, Lvping; Luo, Peng; Zhao, Zhe; Fan, Sigang; Su, Ting] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China; [Wen, Jing; Hu, Chaoqun; Zhang, Lvping; Luo, Peng; Zhao, Zhe; Fan, Sigang; Su, Ting] Chinese Acad Sci, Chinese Acad Sci LAMB, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Wen, Jing; Fan, Sigang; Su, Ting] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
KeywordSea Cucumber Pcr-rflp Fins 16s Rrna Gene Species Identification
Subject AreaFood Science & Technology
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Indexed Bysci
Funding Project海洋地质
WOS IDWOS:000272752800008
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/4354
Collection中科院边缘海地质重点实验室
Corresponding Authorcqhu@scsio.ac.cn
Recommended Citation
GB/T 7714
Wen, J,Hu, CQ,Zhang, L,et al. The application of PCR-RFLP and FINS for species identification used in sea cucumbers (Aspidochirotida: Stichopodidae) products from the market[J]. FOOD CONTROL,2010,21(4):403-407.
APA Wen, J.,Hu, CQ.,Zhang, L.,Luo, P.,Zhao, Z.,...&cqhu@scsio.ac.cn.(2010).The application of PCR-RFLP and FINS for species identification used in sea cucumbers (Aspidochirotida: Stichopodidae) products from the market.FOOD CONTROL,21(4),403-407.
MLA Wen, J,et al."The application of PCR-RFLP and FINS for species identification used in sea cucumbers (Aspidochirotida: Stichopodidae) products from the market".FOOD CONTROL 21.4(2010):403-407.
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