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学科主题: Chemistry, Analytical
题名: Biotransformation of limonene by marine bacteria
作者: Li, HJ ; Lan, WJ ; Cai, CH ; Zhou, YP ; Lin, YC
关键词: marine bacteria ; limonene ; biotransformation
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2006
卷: 34, 期:7, 页:946-950
收录类别: sci
部门归属: Sun Yat Sen Univ, Sch Chem & Chem Engn, Guangzhou 510275, Peoples R China; Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510080, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Peoples R China
项目归属: 海洋生物
摘要: D-Limonene was effectively biotransformed by marine bacteria, Vibrio cholerae, Listonella damsela and Vibrio alginolyticus which collected from the Daya Bay of the South China Sea. These bacterial strains were fermented in the liquid medium 2216E, D- limonene was distributed among the culture flasks (200 mg/L). The flasks were incubated at 28 degrees C on a rotary shaker (r/min = 120) for 5 days. On completion, the culture broth were successively extracted with EtOAc. The extract was quantitatively measured by gas chromatography-mass spectrometry (GC-MS) analysis. The results showed that biocatalytic reactions were comprised of oxidation, including hydroxylation and ketonization which occurred at different positions of the D-limonene, as well as hydrogenation, hydrolyzation, esterification and cyclic cleavage. These bacteria strains possess the distinctive biocatalytic capacities towards the D-limonene. A series of terpenoids, including sesqui-, di- and tri terpenoids were also observed, which was accounted for D-limonene had influence on the metabolites of marine bacteria. Biotransformation is a valuable tool, with the advantages of the simple manipulation, working under mild reaction conditions, high efficiency, being reaction-, regio-, and stereoselective, and friendly. Microorganisms biotransformation definitely represents a new powerful approach to the development and the utilization of monoterpene, the abundant natural resource and provides the endless opportunities to obtain some significant compounds which can be used in the fields of medicine, organic synthesis and fine chemistry.
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WOS记录号: WOS:000242858300011
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/4434
Appears in Collections:中科院边缘海地质重点实验室_期刊论文

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Li, HJ; Lan, WJ; Cai, CH; Zhou, YP; Lin, YC.Biotransformation of limonene by marine bacteria,CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(7):946-950
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