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Isolation and characterization of a carotenoid oxygenase gene from Chlorella zofingiensis (Chlorophyta)
Huang, JC; Wang, Y; Sandmann, G; Chen, F; sfchen@hkusua.hku.hk
2006
发表期刊APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
卷号71期号:4页码:473-479
摘要The green alga Chlorella zofingiensis produces large amounts of the valuable ketocarotenoid astaxanthin under dark, heterotrophic growth conditions, making it potentially employable for commercial production of astaxanthin as feed additives, colorants, and health products. Here, we report the identification and characterization of a beta-carotene oxygenase (CRTO) gene that is directly involved in the biosynthesis of ketocarotenoids in C. zofingiensis. The open reading frame of the crtO gene, which is interrupted by three introns of 243, 318, and 351 bp, respectively, encodes a polypeptide of 312 amino acid residues. Only one crtO gene was detected in the genome of C. zofingiensis. Furthermore, the expression of the crtO gene was transiently up-regulated upon glucose treatment. Functional complementation in Escherichia coli showed that the coding protein of the crtO gene not only exhibits normal CRTO activity by converting beta-carotene to canthaxanthin via echinenone, but also displays a high enzymatic activity of converting zeaxanthin to astaxanthin via adonixanthin. Based on the bifunctional CRTO, a predicted pathway for astaxanthin biosynthesis in C. zofingiensis is described, and the CRTO is termed as carotenoid 4,4'-beta-ionone ring oxygenase.
部门归属Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Peoples R China; Univ Frankfurt, Inst Bot, D-60054 Frankfurt, Germany
学科领域Biotechnology & Applied Microbiology
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收录类别sci
WOS记录号WOS:000239020100013
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/4440
专题中科院边缘海地质重点实验室
通讯作者sfchen@hkusua.hku.hk
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Huang, JC,Wang, Y,Sandmann, G,et al. Isolation and characterization of a carotenoid oxygenase gene from Chlorella zofingiensis (Chlorophyta)[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2006,71(4):473-479.
APA Huang, JC,Wang, Y,Sandmann, G,Chen, F,&sfchen@hkusua.hku.hk.(2006).Isolation and characterization of a carotenoid oxygenase gene from Chlorella zofingiensis (Chlorophyta).APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,71(4),473-479.
MLA Huang, JC,et al."Isolation and characterization of a carotenoid oxygenase gene from Chlorella zofingiensis (Chlorophyta)".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 71.4(2006):473-479.
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