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Morphological, structural and functional characteristics of the hemocytes of the oyster, Crassostrea ariakensis
Sun, JF; Wu, XZ; Zhang, WZ; wuxz@zju.edu.cn
2006
发表期刊JOURNAL OF SHELLFISH RESEARCH
ISSN0730-8000
卷号25期号:1页码:55-64
摘要Light and electron microscopical studies were carried out to characterize the hemocytes of the oyster. Crassostrea ariakensis. Three types of hemocytes were recognized: granulocytes, large hyalinocytes and small hyalinocytes. The large hyalinocytes and small hyalinocytes were granular cells. The large hyalinocytes presented thin cytoplasm and high nucleus: cytoplasm ratios. The small hyalinocytes were the most homogeneous in shape and showed round or oval. They have the highest nucleus: cytoplasm ratios and contained very thin cytoplasm. The granulocytes showed abundant cytoplasm and low nucleus: cytoplasm ratios. At the ultrastructural level, the large hyalinocytes cytoplasm contained a variable number of mitochondria. Golgi complex and endoplasmic reticulum. They showed a total absence of cytoplasmic granules or a few small electron-lucid vesicles of different sizes. The granulocytes showed more polymorphic than the agranular cells, with numerous pseudopodia sprouting off their surface. The granulocytes showed similar organelles, but on the contrary they had abundant electron-dense particles or electron-lucent granules in the cell cytoplasm. The small hyalinocytes presented a total absence of cytoplasmic granules and few organelles and only one or two mitochondria were sometimes observed in the cytoplasm. Total hemocyte Counts gave a mean (SE) concentration of 2.06 +/- 0.20 x 10(7) cells mL(-1) of hemolymph. Differential hemocyte counts identified the granulocytes as the predominant cell type, followed by the large hyalinocytes and the small hyalinocytes. The percentages of the three cell types in the hemocyte population of C. ariakensis were 68.4%, 21.6% and 9.0%, respectively. The quantitative study of phagocytosis showed that the granulocytes were phagocytic cells and the agranular cells showed a limited phagocytic ability. After the separation in Percoll density gradient, the granulocytes were separated from agranulocytes and pure granulocytes were obtained. However, the separation of different agranular cell types (large hyalinocytes and small hyalinocytes) was not achieved by Percoll or Ficoll gradient centrifugation. Our study provides a morphofunctional basis for the cellular defense mechanisms in C. ariakensis.
部门归属Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; Zhejiang Univ, Coll Anim Sci, Hangzhou 310029, Peoples R China; S China Normal Univ, Coll Life Sci, Guangzhou 510631, Guangdong, Peoples R China
关键词Crassostrea Ariakensis Hemocytes Granulocytes Hyalinocytes Phagocytosis Separation
学科领域Fisheries ; Marine & Freshwater Biology
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收录类别sci
WOS记录号WOS:000237149500007
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/4444
专题中科院边缘海地质重点实验室
通讯作者wuxz@zju.edu.cn
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Sun, JF,Wu, XZ,Zhang, WZ,et al. Morphological, structural and functional characteristics of the hemocytes of the oyster, Crassostrea ariakensis[J]. JOURNAL OF SHELLFISH RESEARCH,2006,25(1):55-64.
APA Sun, JF,Wu, XZ,Zhang, WZ,&wuxz@zju.edu.cn.(2006).Morphological, structural and functional characteristics of the hemocytes of the oyster, Crassostrea ariakensis.JOURNAL OF SHELLFISH RESEARCH,25(1),55-64.
MLA Sun, JF,et al."Morphological, structural and functional characteristics of the hemocytes of the oyster, Crassostrea ariakensis".JOURNAL OF SHELLFISH RESEARCH 25.1(2006):55-64.
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