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学科主题: Biotechnology & Applied Microbiology; Environmental Sciences
题名: Degradation of p-nitrophenol by Achromobacter xylosoxidans Ns isolated from wetland sediment
作者: Wan, N ; Gu, JD ; Yan, Y
通讯作者: jdgu@hkucc.hku.hk
关键词: biodegradation ; p-nitrophenol ; 3-nitrophenol ; Achroinobacter xylosoxidans ; wetland
刊名: INTERNATIONAL BIODETERIORATION & BIODEGRADATION
发表日期: 2007
卷: 59, 期:2, 页:90-96
收录类别: sci
部门归属: Univ Hong Kong, Dept Ecol & Biodivers, Lab Environm Microbiol & Toxicol, Hong Kong, Hong Kong, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanog, Guangzhou 510301, Peoples R China
项目归属: 海洋地质
摘要: Achromobacter xylosoxidans Ns strain, capable of utilizing p-nitrophenol (PNP) as the sole source of carbon, energy, and nitrogen, was isolated from wetland sediment and confirmed based on 16S rRNA gene sequence. The strain Ns could tolerate concentrations of PNP up to 1.8mM, and degradation of PNP was achieved in 7d at 30 degrees C in the dark under aerobic conditions. Biodegradation of PNP occurred quickly at an optimal pH of 7.0 and higher, and at <= 0.5% salt (NaCl) contents. During bacterial growth on PNP, 4-nitrocatechol was observed as a key degradation intermediate using a combination of techniques, including HPLC, UV-visible spectra, and comparison with the authentic standard. In a similar way, a second degradation intermediate was identified to be 1,2,4-benzenetriol. Moreover, A. xylosoxidans Ns could also degrade 3-nitrophenol as the sole source of carbon, nitrogen, and energy, but 2-nitrophenol could not. The experimental results showed that bacteria indigenous to the wetland sediment are capable of degradading PNP and chemicals with similar structures. (c) 2006 Elsevier Ltd. All rights reserved.
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WOS记录号: WOS:000244805600003
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/4508
Appears in Collections:中科院边缘海地质重点实验室_期刊论文

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Wan, N; Gu, JD; Yan, Y.Degradation of p-nitrophenol by Achromobacter xylosoxidans Ns isolated from wetland sediment,INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2007,59(2):90-96
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文件名: Degradation of p-nitrophenol by Achromobacter xylosoxidans Ns isolated from wetland sediment .pdf
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