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Production of tetraploid pearl oyster (Pinctada martensii Dunker) by inhibiting the first polar body in eggs from triploids
He, MX; Lin, YG; Shen, Q; Hu, JX; Jiang, WG; He, MX, Chinese Acad Sci, S China Sea Inst Oceanol, 164 W Xingang Rd, Guangzhou 510301, Peoples R China
2000
发表期刊JOURNAL OF SHELLFISH RESEARCH
ISSN0730-8000
卷号19期号:1页码:147-151
摘要All previous attempts to produce viable tetraploid pearl oyster (Pinctada martensii Dunker) by inhibiting the first polar body and the first mitosis have failed. This study aims to test the possibility of producing viable tetraploids by the intentional process of crossing triploid females and diploid males following the inhibition of the first polar body. When 0.5mg/L CB was applied to inhibit the release of the first polar body, about 16.69% of embryos developed as tetraploids, the majority of embryos were aneuploids (65.48%); about 82% embryos developed as aneuploids in the control group (TD1), but no tetraploid embryos were found. Ploidy of embryos in the TD1 group mainly fell between 2n and 3n, bur ranged from 2n to 5n in the TDCB group. During rearing period, larvae died heavily. At Day 51 post-fertilization, 2125 spat were harvested, averaging 0.033% of D-larvae cultured. Chromosome analysis revealed that 115 one-year-old pearl oysters consisted of 28.70% diploids (n = 33), 40.87% triploids (n = 47), 1.74% tetraploids (n = 2) and 28.70% aneuploids (n = 33) with 29, 30, 40, 41, and 43 chromosomes. Comparison of growth showed that aneuploids was not significantly different from diploids in both shell length and body weight (P > 0.1), but significantly smaller than triploids (P < 0.05). This study demonstrated that the production of viable tetraploid pearl oysters with eggs from triploids is possible, and certain levels of aneuploidy can be tolerated in this species.
部门归属Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Peoples R China
关键词Tetraploid Aneuploid Triploid PinctaD. Martensii (D.)
学科领域Fisheries ; Marine & Freshwater Biology
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收录类别sci
资助项目海洋地质
WOS记录号WOS:000088636000026
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被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/4674
专题中科院边缘海地质重点实验室
通讯作者He, MX, Chinese Acad Sci, S China Sea Inst Oceanol, 164 W Xingang Rd, Guangzhou 510301, Peoples R China
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GB/T 7714
He, MX,Lin, YG,Shen, Q,et al. Production of tetraploid pearl oyster (Pinctada martensii Dunker) by inhibiting the first polar body in eggs from triploids[J]. JOURNAL OF SHELLFISH RESEARCH,2000,19(1):147-151.
APA He, MX,Lin, YG,Shen, Q,Hu, JX,Jiang, WG,&He, MX, Chinese Acad Sci, S China Sea Inst Oceanol, 164 W Xingang Rd, Guangzhou 510301, Peoples R China.(2000).Production of tetraploid pearl oyster (Pinctada martensii Dunker) by inhibiting the first polar body in eggs from triploids.JOURNAL OF SHELLFISH RESEARCH,19(1),147-151.
MLA He, MX,et al."Production of tetraploid pearl oyster (Pinctada martensii Dunker) by inhibiting the first polar body in eggs from triploids".JOURNAL OF SHELLFISH RESEARCH 19.1(2000):147-151.
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