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学科主题: Fisheries; Marine & Freshwater Biology
题名: Production of tetraploid pearl oyster (Pinctada martensii Dunker) by inhibiting the first polar body in eggs from triploids
作者: He, MX ; Lin, YG ; Shen, Q ; Hu, JX ; Jiang, WG
通讯作者: He, MX, Chinese Acad Sci, S China Sea Inst Oceanol, 164 W Xingang Rd, Guangzhou 510301, Peoples R China
关键词: tetraploid ; aneuploid ; triploid ; Pinctada martensii (D.)
刊名: JOURNAL OF SHELLFISH RESEARCH
发表日期: 2000
卷: 19, 期:1, 页:147-151
收录类别: sci
部门归属: Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Peoples R China
项目归属: 海洋地质
摘要: All previous attempts to produce viable tetraploid pearl oyster (Pinctada martensii Dunker) by inhibiting the first polar body and the first mitosis have failed. This study aims to test the possibility of producing viable tetraploids by the intentional process of crossing triploid females and diploid males following the inhibition of the first polar body. When 0.5mg/L CB was applied to inhibit the release of the first polar body, about 16.69% of embryos developed as tetraploids, the majority of embryos were aneuploids (65.48%); about 82% embryos developed as aneuploids in the control group (TD1), but no tetraploid embryos were found. Ploidy of embryos in the TD1 group mainly fell between 2n and 3n, bur ranged from 2n to 5n in the TDCB group. During rearing period, larvae died heavily. At Day 51 post-fertilization, 2125 spat were harvested, averaging 0.033% of D-larvae cultured. Chromosome analysis revealed that 115 one-year-old pearl oysters consisted of 28.70% diploids (n = 33), 40.87% triploids (n = 47), 1.74% tetraploids (n = 2) and 28.70% aneuploids (n = 33) with 29, 30, 40, 41, and 43 chromosomes. Comparison of growth showed that aneuploids was not significantly different from diploids in both shell length and body weight (P > 0.1), but significantly smaller than triploids (P < 0.05). This study demonstrated that the production of viable tetraploid pearl oysters with eggs from triploids is possible, and certain levels of aneuploidy can be tolerated in this species.
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WOS记录号: WOS:000088636000026
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/4674
Appears in Collections:中科院边缘海地质重点实验室_期刊论文

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Recommended Citation:
He, MX; Lin, YG; Shen, Q; Hu, JX; Jiang, WG.Production of tetraploid pearl oyster (Pinctada martensii Dunker) by inhibiting the first polar body in eggs from triploids,JOURNAL OF SHELLFISH RESEARCH,2000,19(1):147-151
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