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Vaccination of the grouper, Epinephalus awoara, against vibriosis using the ultrasonic technique
Zhou, YC; Huang, H; Wang, J; Zhang, B; Su, YQ
2002
发表期刊AQUACULTURE
ISSN0044-8486
卷号203期号:40606页码:229-238
摘要A novel ultrasonic technique was used to facilitate the vaccination of fish against Vibrio alginolyticus. To establish the safety parameters, we first tested the effects of ultrasound treatment on juvenile groupers, Epinephalus awoara. Results showed that, at an intensity of 400 mW/cm(2), 10 min of ultrasound treatment were safe, whereas an ultrasound intensity of 600 mW/cm(2) produced a certain degree of damage to the experimental groupers. The ultrasound frequency had little effect on the survival of the treated fish. Next we determined the protective effect of the ultrasound-facilitated vaccination. A low frequency ultrasound (35 kHz) with an intensity of 175 mW/cm(2) was used for vaccinating fish against vibriosis. Different ultrasonic vaccination methods were examined; each contains a total of 2-min continuous or pulsed ultrasound combined with or without 2-min immersion in the presence of vibriosis vaccine. Of all the eight ultrasonic inoculating methods tested, 'pulsed ultrasound followed by immersion' and 'immersion, pulsed ultrasound and immersion again' provided the best protection from bacterial challenge. Compared to other traditional methods, the protective effect provided by ultrasonic vaccination is comparable to that by the intraperitoneal injection method, and the operation convenience is comparable to that by the immersion method. Thus, the ultrasound-facilitated vaccination provides an effective and practical approach for vaccinating fish on large scale. (C) 2002 Elsevier Science B.V. All rights reserved.
部门归属Hainan Univ, Dept Fisheries, Haikou 570228, Peoples R China; Xiamen Univ, Dept Oceanog, Xiamen 361005, Peoples R China; CAS, S China Sea Inst Oceanol, Guangzhou 510301, Peoples R China
关键词Epinephalus Awoara Vibrio Alginolyticus Vaccination Ultrasound
学科领域Fisheries ; Marine & Freshwater Biology
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收录类别sci
资助项目海洋地质
WOS记录号WOS:000173122100002
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被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/4730
专题中科院边缘海地质重点实验室
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Zhou, YC,Huang, H,Wang, J,et al. Vaccination of the grouper, Epinephalus awoara, against vibriosis using the ultrasonic technique[J]. AQUACULTURE,2002,203(40606):229-238.
APA Zhou, YC,Huang, H,Wang, J,Zhang, B,&Su, YQ.(2002).Vaccination of the grouper, Epinephalus awoara, against vibriosis using the ultrasonic technique.AQUACULTURE,203(40606),229-238.
MLA Zhou, YC,et al."Vaccination of the grouper, Epinephalus awoara, against vibriosis using the ultrasonic technique".AQUACULTURE 203.40606(2002):229-238.
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