SCSIO OpenIR  > 中科院边缘海地质重点实验室
Vaccination of the grouper, Epinephalus awoara, against vibriosis using the ultrasonic technique
Zhou, YC; Huang, H; Wang, J; Zhang, B; Su, YQ
2002
Source PublicationAQUACULTURE
ISSN0044-8486
Volume203Issue:40606Pages:229-238
AbstractA novel ultrasonic technique was used to facilitate the vaccination of fish against Vibrio alginolyticus. To establish the safety parameters, we first tested the effects of ultrasound treatment on juvenile groupers, Epinephalus awoara. Results showed that, at an intensity of 400 mW/cm(2), 10 min of ultrasound treatment were safe, whereas an ultrasound intensity of 600 mW/cm(2) produced a certain degree of damage to the experimental groupers. The ultrasound frequency had little effect on the survival of the treated fish. Next we determined the protective effect of the ultrasound-facilitated vaccination. A low frequency ultrasound (35 kHz) with an intensity of 175 mW/cm(2) was used for vaccinating fish against vibriosis. Different ultrasonic vaccination methods were examined; each contains a total of 2-min continuous or pulsed ultrasound combined with or without 2-min immersion in the presence of vibriosis vaccine. Of all the eight ultrasonic inoculating methods tested, 'pulsed ultrasound followed by immersion' and 'immersion, pulsed ultrasound and immersion again' provided the best protection from bacterial challenge. Compared to other traditional methods, the protective effect provided by ultrasonic vaccination is comparable to that by the intraperitoneal injection method, and the operation convenience is comparable to that by the immersion method. Thus, the ultrasound-facilitated vaccination provides an effective and practical approach for vaccinating fish on large scale. (C) 2002 Elsevier Science B.V. All rights reserved.
DepartmentHainan Univ, Dept Fisheries, Haikou 570228, Peoples R China; Xiamen Univ, Dept Oceanog, Xiamen 361005, Peoples R China; CAS, S China Sea Inst Oceanol, Guangzhou 510301, Peoples R China
KeywordEpinephalus Awoara Vibrio Alginolyticus Vaccination Ultrasound
Subject AreaFisheries ; Marine & Freshwater Biology
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Indexed Bysci
Funding Project海洋地质
WOS IDWOS:000173122100002
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/4730
Collection中科院边缘海地质重点实验室
Recommended Citation
GB/T 7714
Zhou, YC,Huang, H,Wang, J,et al. Vaccination of the grouper, Epinephalus awoara, against vibriosis using the ultrasonic technique[J]. AQUACULTURE,2002,203(40606):229-238.
APA Zhou, YC,Huang, H,Wang, J,Zhang, B,&Su, YQ.(2002).Vaccination of the grouper, Epinephalus awoara, against vibriosis using the ultrasonic technique.AQUACULTURE,203(40606),229-238.
MLA Zhou, YC,et al."Vaccination of the grouper, Epinephalus awoara, against vibriosis using the ultrasonic technique".AQUACULTURE 203.40606(2002):229-238.
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