中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室  > 期刊论文
学科主题: Biotechnology & Applied Microbiology; Microbiology
题名: Identification and pathogenicity of Vibrio ponticus affecting cultured Japanese sea bass, Lateolabrax japonicus (Cuvier in Cuvier and Valenciennes)
作者: Xie, ZY ; Hu, CQ ; Zhang, LP ; Chen, C ; Ren, CH ; Shen, Q
通讯作者: cqhu@scsio.ac.cn
关键词: denaturing gradient gel electrophoresis ; epidemiologic investigation ; Lateolabrax japonicas ; pathogenicity ; Vibrio ponticus
刊名: LETTERS IN APPLIED MICROBIOLOGY
发表日期: 2007
卷: 45, 期:1, 页:62-67
收录类别: sci
部门归属: Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Peoples R China
项目归属: LMB
摘要: Aims: To rapidly determine the causative agent of mass death in Lateolabrax japonicus in Zhelin Bay of Guangdong Province in China in April 2004. Methods and Results: Thirty-six strains, numbered sequentially from RP01 to RP36, were isolated from six diseased fish. All of the strains were identified as being of the same vibrio species according to the results of universal primer PCR combined with DGGE (UPPCR-DGGE). RP30 was one of these strains that was randomly selected and analysed by using a morphological, physiological and biochemical plate, Biolog GN2 Microplate System and API 20E system. Furthermore, RP30' 16S rDNA was sequenced and aligned in Genbank. Its virulence to Lateolabrax japonicus (Cuvier in Cuvier and Valenciennes) was also tested. RP30 is most closely related to four Vibrio ponticus strains (99.3% similarity). LD(50)s were 2.5 (x103 CFU per fish for intraperitoneal inoculation (IP) and 3.2 (x103 CFU per fish for intramuscular inoculation (IM), respectively. Conclusions: The investigated pathogenic agent of Lateolabrax japonicus (Cuvier in Cuvier and Valenciennes) was V. ponticus. Significance and Impact of the Study: UPPCR-DGGE is very helpful in epidemiologic investigation. Interestingly, this is the first report that V. ponticus infects cultured marine fish. DGGE was likewise first introduced to epidemiologic investigation of fish disease.
原文出处: 查看原文
WOS记录号: WOS:000247561700011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/4922
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Xie, ZY; Hu, CQ; Zhang, LP; Chen, C; Ren, CH; Shen, Q.Identification and pathogenicity of Vibrio ponticus affecting cultured Japanese sea bass, Lateolabrax japonicus (Cuvier in Cuvier and Valenciennes),LETTERS IN APPLIED MICROBIOLOGY,2007,45(1):62-67
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Xie, ZY]'s Articles
[Hu, CQ]'s Articles
[Zhang, LP]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Xie, ZY]‘s Articles
[Hu, CQ]‘s Articles
[Zhang, LP]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace